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Monitoring fitness and productivity in cyanobacteria batch cultures

SYSNO ASEP	0543490
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Monitoring fitness and productivity in cyanobacteria batch cultures
Author(s)	Zavřel, Tomáš (UEK-B)_{RID, SAI, ORCID} Schoffman, H. (IL) Lukeš, Martin (MBU-M)_ORCID Fedorko, Jan (UEK-B)_{SAI, ORCID} Keren, N. (IL) Červený, Jan (UEK-B)_{RID, ORCID, SAI}
Number of authors	6
Article number	102328
Source Title	Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264 Roč. 56, JUN (2021)
Number of pages	15 s.
Language	eng - English
Country	NL - Netherlands
Keywords	sp pcc 6803 ; photosystem-ii ; microalgae cultivation ; dark respiration ; growth-rate ; light ; phytoplankton ; photosynthesis ; fluorescence ; algae ; Batch cultivation ; Cyanobacteria ; Excitation-emission spectra ; Fitness ; Metal uptake ; Photosynthesis
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
Subject RIV - cooperation	Institute of Microbiology - Microbiology, Virology
R&D Projects	LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA18-24397S GA ČR - Czech Science Foundation (CSF)
	EF16_026/0008413 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
Research Infrastructure	CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i.
Method of publishing	Limited access
Institutional support	UEK-B - RVO:86652079 ; MBU-M - RVO:61388971
UT WOS	000654306500008
EID SCOPUS	85108537722
DOI	10.1016/j.algal.2021.102328
Annotation	Cyanobacteria are key photosynthetic organisms in many aquatic ecosystems and hold great potential for sustainable green biotechnology. Growth of cyanobacteria in batch cultures is expected to be part of future biotechnological practices. However, the issue of correlating the dynamics of metabolic and photosynthetic parameters with the culture fitness during batch cultivation is still outstanding. In this paper we take advantage of a photobioreactor system to continuously track growth parameters of Synechocystis sp. PCC 6803, and to couple online culture monitoring with offline measurements of photosynthetic efficiency and biochemical and elemental cell composition under several light intensity and CO2 regimes. Light intensity determines the flux of energy into the photosynthetic system while CO2 concentrations determines the ability to capture this energy in chemical form. From this perspective, four distinct source-sink regimes were established and compared, which allowed us to reveal specific strategies to acclimate to both carbon and light limitation. As part of the measurements, room temperature excitation-emission spectra and elemental composition of Synechocystis cells were, for the first time, compared throughout the exponential and linear growth phases. In total, 39 parameters (out of 170 measured) were identified as highly correlating (R2 > 0.9) with growth rate or productivity under at least one tested cultivation condition, including concentrations and ratios of pigments or particular elements. For online fitness and productivity monitoring in cyanobacteria batch cultures, parameters such as photosynthesis and respiration rates and ratios, energy-dependent non-photochemical quenching (qE) or Zn and Mo concentration in the cultivation medium can be of interest.
Workplace	Global Change Research Institute
Contact	Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
Year of Publishing	2022
Electronic address	https://www.sciencedirect.com/science/article/pii/S2211926421001478?via%3Dihub

Number of the records: 1