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Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor
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SYSNO ASEP 0543466 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor Author(s) Ivanov, Ivan (MBU-M) RID
Zachleder, Vilém (MBU-M) ORCID
Vítová, Milada (MBU-M) RID, ORCID
Barbosa, M- J. (NL)
Bišová, Kateřina (MBU-M) RIDArticle number 1084 Source Title Cells. - : MDPI
Roč. 10, č. 5 (2021)Number of pages 14 s. Language eng - English Country CH - Switzerland Keywords microalgae ; Chlamydomonas reinhardtii ; starch ; supraoptimal temperature ; cell cycle ; pilot-scale production Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA15-09231S GA ČR - Czech Science Foundation (CSF) EF16_027/0007990 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000654688700001 EID SCOPUS 85107427352 DOI 10.3390/cells10051084 Annotation An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39 degrees C) causes a complete block of nuclear and cellular division accompanied by an increased accumulation of starch. In this work we explore the potential of supraoptimal temperature as a method to promote starch production in C. reinhardtii in a pilot-scale photobioreactor. The method was successfully applied and resulted in an almost 3-fold increase in the starch content of C. reinhardtii dry matter. Moreover, a maximum starch content at the supraoptimal temperature was reached within 1-2 days, compared with 5 days for the control culture at the optimal temperature (30 degrees C). Therefore, supraoptimal temperature treatment promotes rapid starch accumulation and suggests a viable alternative to other starch-inducing methods, such as nutrient depletion. Nevertheless, technical challenges, such as bioreactor design and light availability within the culture, still need to be dealt with. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2073-4409/10/5/1084
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