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Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography
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SYSNO ASEP 0543192 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography Author(s) Vítová, Milada (MBU-M) RID, ORCID
Stránská, M. (CZ)
Palyzova, A. (CZ)
Řezanka, Tomáš (MBU-M) ORCIDArticle number 462185 Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1648, JUL 5 2021 (2021)Number of pages 14 s. Language eng - English Country NL - Netherlands Keywords Enantiomeric separation ; Enzymatic hydrolysis ; rp-hplc/ms-esi+ ; Cardiolipins ; Bacterium ; Yeast ; Alga ; Spinach ; Bovine heart Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000649591700003 EID SCOPUS 85105575127 DOI 10.1016/j.chroma.2021.462185 Annotation Cardiolipins (1,3-bis(sn-3'-phosphatidyl)-sn-glycerol) (CLs) are widespread in many organisms, from bacteria to higher green plants and mammals. CLs were observed in Gram-positive bacterium of the genus Kocuria, brewer's yeast Saccharomyces, the green alga Chlamydomonas, spinach and beef heart. A mixture of molecular species of CLs was obtained from total lipids by hydrophilic interaction liquid chromatography (HILIC), and these were further separated and identified by reversed phase LC/MS with negative tandem electrospray ionization. The majority of CLs molecular species from each organism were cleaved using phospholipase C from Bacillus cereus. This phospholipase cleaves CLs into 1,2-diglycerols and phosphatidylglycerol 3-phosphates, which were then separated. After CLs cleavage, diacylglycerols such as sn-1,2-diacyl-3-acetyl-glycerols (i.e., triacylglycerols) were separated and identified by chiral chromatography/MS-positive tandem ESI. Significant differences in the composition of the molecular species between the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of CLs were found in all organisms tested. Molecular species of CLs that contained four different fatty acids were identified in all five samples, and CLs containing very long chain fatty acids were identified in yeast. In addition, CLs containing both enantiomers (at the sn-2 carbon) were present in the bacterium tested. These findings were further supported by data already published in GenBank where, in the same family Micrococ-caceae both enzymes responsible for chirality in the sn-2 position, glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases, were present. (C) 2021 Elsevier B.V. All rights reserved. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0021967321003095
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