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Dehydroflavonolignans from Silymarin Potentiate Transition Metal Toxicity In Vitro but Are Protective for Isolated Erythrocytes Ex Vivo
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SYSNO ASEP 0543091 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dehydroflavonolignans from Silymarin Potentiate Transition Metal Toxicity In Vitro but Are Protective for Isolated Erythrocytes Ex Vivo Author(s) Lomozová, Z. (CZ)
Tvrdý, V. (CZ)
Hrubša, M. (CZ)
Catapano, M.C. (CZ)
Macáková, K. (CZ)
Biedermann, David (MBU-M) RID, ORCID
Kučera, R. (CZ)
Křen, Vladimír (MBU-M) RID, ORCID
Mladěnka, P. (CZ)
Valentová, Kateřina (MBU-M) RID, ORCIDArticle number 679 Source Title Antioxidants. - : MDPI
Roč. 10, č. 5 (2021)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords flavonolignans ; silymarin ; milk thistle ; dehydroflavonolignans ; dehydrosilybin ; dehydrosilychristin ; hydroxyl radical ; iron ; copper ; prooxidation Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA18-00121S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000653378600001 EID SCOPUS 85104801813 DOI 10.3390/antiox10050679 Annotation 2,3-Dehydrosilybin (DHS) was previously shown to chelate and reduce both copper and iron ions. In this study, similar experiments with 2,3-dehydrosilychristin (DHSCH) showed that this congener of DHS also chelates and reduces both metals. Statistical analysis pointed to some differences between both compounds: in general, DHS appeared to be a more potent iron and copper chelator, and a copper reducing agent under acidic conditions, while DHSCH was a more potent copper reducing agent under neutral conditions. In the next step, both DHS and DHSCH were tested for metal-based Fenton chemistry in vitro using HPLC with coulometric detection. Neither of these compounds were able to block the iron-based Fenton reaction and, in addition, they mostly intensified hydroxyl radical production. In the copper-based Fenton reaction, the effect of DHSCH was again prooxidant or neutral, while the effect of DHS was profoundly condition-dependent. DHS was even able to attenuate the reaction under some conditions. Interestingly, both compounds were strongly protective against the copper-triggered lysis of red blood cells, with DHSCH being more potent. The results from this study indicated that, notwithstanding the prooxidative effects of both dehydroflavonolignans, their in vivo effect could be protective. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2076-3921/10/5/679
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