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Tissue clearing and imaging methods for cardiovascular development
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SYSNO ASEP 0542872 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tissue clearing and imaging methods for cardiovascular development Author(s) Kolesová, Hana (FGU-C) RID, ORCID, SAI
Olejníčková, Veronika (FGU-C) RID, ORCID, SAI
Kvasilová, A. (CZ)
Gregorovičová, Martina (FGU-C) RID, ORCID, SAI
Sedmera, David (FGU-C) RID, ORCID, SAIArticle number 102387 Source Title iScience. - : Cell Press
Roč. 24, č. 4 (2021)Number of pages 25 s. Language eng - English Country US - United States Keywords tissue clearing ; imaging ; cardiovascular development Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery OECD category Cardiac and Cardiovascular systems R&D Projects LTC17023 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-03207S GA ČR - Czech Science Foundation (CSF) GA18-03461S GA ČR - Czech Science Foundation (CSF) GA21-03847S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000642261700126 EID SCOPUS 85104350157 DOI 10.1016/j.isci.2021.102387 Annotation Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different tissue clearing methods for 3D imaging of heart and vasculature, based on chemical composition (solvent-based, simple immersion, hyperhydration, and hydrogel embedding techniques). We discuss in detail compatibility of various tissue clearing techniques with visualization methods: fluorescence preservation, immunohistochemistry, nuclear staining, and fluorescent dyes vascular perfusion. We also discuss myocardium visualization using autofluorescence, tissue shrinking, and expansion. Then we overview imaging methods used to study cardiovascular system and live imaging. We discuss heart and vessels segmentation methods and image analysis. The review covers the whole process of cardiovascular system 3D imaging, starting from tissue clearing and its compatibility with various visualization methods to the types of imaging methods and resulting image analysis. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.isci.2021.102387
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