Number of the records: 1

Degradation Products of Polychlorinated Biphenyls and Their In Vitro Transformation by Ligninolytic Fungi

SYSNO ASEP	0542795
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Degradation Products of Polychlorinated Biphenyls and Their In Vitro Transformation by Ligninolytic Fungi
Author(s)	Šrédlová, Kamila (MBU-M)_ORCID Šírová, Kateřina (MBU-M) Stella, Tatiana (MBU-M) Cajthaml, Tomáš (MBU-M)_{RID, ORCID}
Article number	81
Source Title	Toxics. - : MDPI Roč. 9, č. 4 (2021)
Number of pages	15 s.
Language	eng - English
Country	CH - Switzerland
Keywords	hydroxylated PCBs ; chlorobenzyl alcohols ; chlorobenzaldehydes ; biodegradation ; Pleurotus ostreatus ; Irpex lacteus
Subject RIV	EE - Microbiology, Virology
OECD category	Environmental sciences (social aspects to be 5.7)
Subject RIV - cooperation	Institute of Microbiology - Water Pollution ; Quality
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971
UT WOS	000643628200001
EID SCOPUS	85104856938
DOI	10.3390/toxics9040081
Annotation	Metabolites of polychlorinated biphenyls (PCBs)-hydroxylated PCBs (OH-PCBs), chlorobenzyl alcohols (CB-OHs), and chlorobenzaldehydes (CB-CHOs)-were incubated in vitro with the extracellular liquid of Pleurotus ostreatus, which contains mainly laccase and low manganese-dependent peroxidase (MnP) activity. The enzymes were able to decrease the amount of most of the tested OH-PCBs by > 80% within 1 h, the removal of more recalcitrant OH-PCBs was greatly enhanced by the addition of the laccase mediator syringaldehyde. Conversely, glutathione substantially hindered the reaction, suggesting that it acted as a laccase inhibitor. Hydroxylated dibenzofuran and chlorobenzoic acid were identified as transformation products of OH-PCBs. The extracellular enzymes also oxidized the CB-OHs to the corresponding CB-CHOs on the order of hours to days, however, the mediated and nonmediated setups exhibited only slight differences, and the participating enzymes could not be determined. When CB-CHOs were used as the substrates, only partial transformation was observed. In an additional experiment, the extracellular liquid of Irpex lacteus, which contains predominantly MnP, was able to efficiently transform CB-CHOs with the aid of glutathione, mono- and di-chloroacetophenones were detected as transformation products. These results demonstrate that extracellular enzymes of ligninolytic fungi can act on a wide range of PCB metabolites, emphasizing their potential for bioremediation.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2305-6304/9/4/81

Number of the records: 1