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How the molecular weight affects the in vivo fate of exogenous hyaluronan delivered intravenously: A stable-isotope labelling strategy
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SYSNO ASEP 0542790 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title How the molecular weight affects the in vivo fate of exogenous hyaluronan delivered intravenously: A stable-isotope labelling strategy Author(s) Simek, M. (CZ)
Nešporová, K. (CZ)
Kocurková, Anna (BFU-R) ORCID
Foglova, T. (CZ)
Ambrožová, Gabriela (BFU-R) RID
Velebný, V. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCID
Hermannová, M. (CZ)Number of authors 8 Article number 117927 Source Title Carbohydrate Polymers. - : Elsevier - ISSN 0144-8617
Roč. 263, JUL 1 2021 (2021)Number of pages 10 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords chondroitin sulfate ; acid ; size ; pharmacokinetics ; biodistribution Subject RIV CC - Organic Chemistry OECD category Organic chemistry Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000640429500006 EID SCOPUS 85103127618 DOI 10.1016/j.carbpol.2021.117927 Annotation There is inconsistent information regarding the size effects of exogenously given hyaluronan on its in vivo fate. The data are often biased by the poor quality of hyaluronan and non-ideal labelling strategies used for resolving exogenous/endogenous hyaluronan, which only monitor the label and not hyaluronan itself. To overcome these drawbacks and establish the pharmacokinetics of intravenous hyaluronan in relation to its Mw, 13C-labelled HA of five Mws from 13.6?1562 kDa was prepared and administered to mice at doses 25-50 mg kg?1. The elimination efficiency increased with decreasing Mw. Low Mw hyaluronan was rapidly eliminated as small hyaluronan fragments in urine, while high Mw hyaluronan exhibited saturable kinetics and complete metabolization within 48 h. All tested Mws exhibited a similar uptake by liver cells and metabolization into activated sugars. 13Clabelling combined with LC?MS provides an excellent approach to elucidating in vivo fate and biological activities of hyaluronan. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://reader.elsevier.com/reader/sd/pii/S0144861721003143?token=3B1E30F1EFFA8B688701DCC15BDDC999A5E187E791E15A519C7A4D380A7A798486099FF4C0AB22482C63D972D480C238&originRegion=eu-west-1&originCreation=20210528071650
Number of the records: 1