Number of the records: 1

Biocompatible polypeptide nanogel: effect of surfactants on nanogelation in inverse miniemulsion, in vivo biodistribution and blood clearance evaluation

SYSNO ASEP	0542700
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Biocompatible polypeptide nanogel: effect of surfactants on nanogelation in inverse miniemulsion, in vivo biodistribution and blood clearance evaluation
Author(s)	Oleshchuk, Diana (UMCH-V)_ORCID Šálek, Petr (UMCH-V)_{RID, ORCID} Dvořáková, Jana (UMCH-V)_{RID, ORCID} Kučka, Jan (UMCH-V)_{RID, ORCID} Pavlova, Ewa (UMCH-V)_RID Francová, P. (CZ) Šefc, L. (CZ) Proks, Vladimír (UMCH-V)_{RID, ORCID}
Article number	111865
Source Title	Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931 Roč. 126, July (2021)
Number of pages	9 s.
Language	eng - English
Country	NL - Netherlands
Keywords	blood clearance ; inverse miniemulsion ; in vivo biodistribution
Subject RIV	JJ - Other Materials
OECD category	Nano-materials (production and properties)
R&D Projects	GA18-03224S GA ČR - Czech Science Foundation (CSF)
	GA18-07983S GA ČR - Czech Science Foundation (CSF)
Research Infrastructure	Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000663454800004
EID SCOPUS	85106371851
DOI	10.1016/j.msec.2021.111865
Annotation	Horseradish peroxidase (HRP)/H2O2-mediated crosslinking of polypeptides in inverse miniemulsion is a promising approach for the development of next-generation biocompatible and biodegradable nanogels. Herein, we present a fundamental investigation of the effects of three surfactants and their different concentrations on the (HRP)/H2O2-mediated nanogelation of poly[N5-(2-hydroxyethyl)-l-glutamine-ran-N5-propargyl-l-glutamine-ran-N5-(6-aminohexyl)-l-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-l-glutamine] (PHEG-Tyr) in inverse miniemulsion. The surfactants sorbitan monooleate (SPAN 80), polyoxyethylenesorbitan trioleate (TWEEN 85), and dioctyl sulfosuccinate sodium salt (AOT) were selected and their influence on the nanogel size, size distribution, and morphology was evaluated. The most effective nanogelation stabilization was achieved with 20 wt% nonionic surfactant SPAN 80. The diameter of the hydrogel nanoparticles was 230 nm (dynamic light scattering, DLS) and was confirmed also by nanoparticle tracking analysis (NTA) which showed the diameters ranging from 200 to 300 nm. Microscopy and image analyses showed that the nanogel in the dry state was spherical in shape and had number-average diameter Dn = 26 nm and dispersity Ð = 1.91. In the frozen-hydrated state, the nanogel appeared porous and was larger in size with Dn = 182 nm and Ð = 1.52. Our results indicated that the nanogelation of the polymer precursor required a higher concentration of surfactant than classical inverse miniemulsion polymerization to ensure effective stabilization. The developed polypeptide nanogel was radiolabeled with 125I, and in vivo biodistribution and blood clearance evaluations were performed. We found that the 125I-labeled nanogel was well-biodistributed in the bloodstream, cleared from mouse blood during 48 h by renal and hepatic pathways and did not provoke any sign of toxic effects.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2022
Electronic address	https://www.sciencedirect.com/science/article/pii/S0928493121000035?via%3Dihub

Number of the records: 1