Number of the records: 1

K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells

SYSNO ASEP	0542676
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells
Author(s)	Zimmermannová, Olga (FGU-C)_{RID, ORCID} Felcmanová, Kristina (FGU-C) Sácká, Lenka (FGU-C) Colinet, A. S. (BE) Morsomme, P. (BE) Sychrová, Hana (FGU-C)_{RID, ORCID}
Article number	foab015
Source Title	FEMS Yeast Research. - : Oxford University Press - ISSN 1567-1356 Roč. 21, č. 3 (2021)
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	potassium ; calcium ; K+-transporter ; osmotic shock ; Trk1 ; Trk2 ; yeast
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	GA20-04420S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	FGU-C - RVO:67985823
UT WOS	000654733000002
EID SCOPUS	85104047228
DOI	10.1093/femsyr/foab015
Annotation	The maintenance of K+ and Ca2+ homeostasis is crucial for many cellular functions. Potassium is accumulated in cells at high concentrations, while the cytosolic level of calcium, to ensure its signalling function, is kept at low levels and transiently increases in response to stresses. We examined Ca2+ homeostasis and Ca2+ signalling in Saccharomyces cerevisiae strains lacking plasma-membrane K+ influx (Trk1 and Trk2) or efflux (Tok1, Nha1 and Ena1-5) systems. The lack of K+ exporters slightly increased the cytosolic Ca2+, but did not alter the Ca2+ tolerance or Ca2+-stress response. In contrast, the K+-importers Trk1 and Trk2 play important and distinct roles in the maintenance of Ca2+ homeostasis. The presence of Trk1 was vital mainly for the growth of cells in the presence of high extracellular Ca2+, whilst the lack of Trk2 doubled steady-state intracellular Ca2+ levels. The absence of both K+ importers highly increased the Ca2+ response to osmotic or CaCl2 stresses and altered the balance between Ca2+ flux from external media and intracellular compartments. In addition, we found Trk2 to be important for the tolerance to high KCl and hygromycin B in cells growing on minimal media. All the data describe new interconnections between potassium and calcium homeostasis in S. cerevisiae.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://doi.org/10.1093/femsyr/foab015

Number of the records: 1