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Complex Response of the Chlorarachniophyte Bigelowiella natans to Iron Availability
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SYSNO ASEP 0542515 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Complex Response of the Chlorarachniophyte Bigelowiella natans to Iron Availability Author(s) Kotabová, Eva (MBU-M) RID, ORCID
Malych, R. (CZ)
Karlusich, J. J. P. (FR)
Kazamia, E. (FR)
Eichner, Meri (MBU-M) ORCID, RID
Mach, J. (CZ)
Lesuisse, E. (FR)
Bowler, Ch. (FR)
Prášil, Ondřej (MBU-M) RID, ORCID
Suták, R. (CZ)Number of authors 10 Article number e00738-20 Source Title mSystems. - : American Society for Microbiology - ISSN 2379-5077
Roč. 6, č. 1 (2021)Number of pages 19 s. Language eng - English Country GB - United Kingdom Keywords Bigelowiella natans ; iron ; metagenomics ; metatranscriptomics ; photosynthesis ; phytoplankton ; proteomics Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA18-07822S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000647691000020 EID SCOPUS 85102237810 DOI 10.1128/mSystems.00738-20 Annotation The productivity of the ocean is largely dependent on iron availability, and marine phytoplankton have evolved sophisticated mechanisms to cope with chronically low iron levels in vast regions of the open ocean. By analyzing the metabarcoding data generated from the Tara Oceans expedition, we determined how the global distribution of the model marine chlorarachniophyte Bigelowiella natans varies across regions with different iron concentrations. We performed a comprehensive proteomics analysis of the molecular mechanisms underpinning the adaptation of B. natans to iron scarcity and report on the temporal response of cells to iron enrichment. Our results highlight the role of phytotransferrin in iron homeostasis and indicate the involvement of CREG1 protein in the response to iron availability. Analysis of the Tara Oceans metagenomes and metatranscriptomes also points to a similar role for CREG1, which is found to be widely distributed among marine plankton but to show a strong bias in gene and transcript abundance toward iron-deficient regions. Our analyses allowed us to define a new subfamily of the CobW domain-containing COG0523 putative metal chaperones which are involved in iron metabolism and are restricted to only a few phytoplankton lineages in addition to B. natans. At the physiological level, we elucidated the mechanisms allowing a fast recovery of PSII photo-chemistry after resupply of iron. Collectively, our study demonstrates that B. natans is well adapted to dynamically respond to a changing iron environment and suggests that CREG1 and COG0523 are important components of iron homeostasis in B. natans and other phytoplankton. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://msystems.asm.org/content/6/1/e00738-20.abstract
Number of the records: 1