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The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence
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SYSNO ASEP 0542484 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence Author(s) Knoppová, Jana (MBU-M) RID
Yu, J. (GB)
Janouškovec, Jan (MBU-M)
Halada, Petr (MBU-M) RID, ORCID
Nixon, P. J. (GB)
Whitelegge, J. P. (GB)
Komenda, Josef (MBU-M) RID, ORCIDArticle number 3733 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 7 (2021)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords photosystem II ; photosynthesis ; chlorophyll-binding proteins Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GX19-29225X GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000638674800001 EID SCOPUS 85103475840 DOI 10.3390/ijms22073733 Annotation Photochemical energy conversion during oxygenic photosynthesis is performed by membrane-embedded chlorophyll-binding protein complexes. The biogenesis and maintenance of these complexes requires auxiliary protein factors that optimize the assembly process and protect nascent complexes from photodamage. In cyanobacteria, several lipoproteins contribute to the biogenesis and function of the photosystem II (PSII) complex. They include CyanoP, CyanoQ, and Psb27, which are all attached to the lumenal side of PSII complexes. Here, we show that the lumenal Ycf48 assembly factor found in the cyanobacterium Synechocystis sp. PCC 6803 is also a lipoprotein. Detailed mass spectrometric analysis of the isolated protein supported by site-directed mutagenesis experiments indicates lipidation of the N-terminal C29 residue of Ycf48 and removal of three amino acids from the C-terminus. The lipobox sequence in Ycf48 contains a cysteine residue at the3 position compared to Leu/Val/Ile residues found in the canonical lipobox sequence. The atypical Ycf48 lipobox sequence is present in most cyanobacteria but is absent in eukaryotes. A possible role for lipoproteins in the coordinated assembly of cyanobacterial PSII is discussed. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/7/3733
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