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The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine

SYSNO ASEP	0542413
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine
Author(s)	Ergang, Peter (FGU-C)_{RID, ORCID} Vagnerová, Karla (FGU-C)_{RID, ORCID} Hermanová, Petra (MBU-M)_ORCID Vodička, Martin (FGU-C)_{RID, ORCID, SAI} Jágr, M. (CZ) Šrůtková, Dagmar (MBU-M)_{ORCID, RID} Dvořáček, V. (CZ) Hudcovic, Tomáš (MBU-M)_{RID, ORCID} Pácha, Jiří (FGU-C)_{RID, ORCID}
Article number	4229
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 8 (2021)
Number of pages	15 s.
Language	eng - English
Country	CH - Switzerland
Keywords	glucocorticoids ; extra-adrenal steroidogenesis ; 11beta-hydroxysteroid dehydrogenase ; intestine ; microbiome ; anti-CD3 antibody
Subject RIV	ED - Physiology
OECD category	Physiology (including cytology)
Subject RIV - cooperation	Institute of Microbiology - Biochemistry
R&D Projects	GA18-02993S GA ČR - Czech Science Foundation (CSF)
	GA21-10845S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; MBU-M - RVO:61388971
UT WOS	000644332500001
EID SCOPUS	85104404619
DOI	10.3390/ijms22084229
Annotation	Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1 beta, IL6 and Tnf alpha and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/8/4229

Number of the records: 1