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Insight into the Mechanism of Action and Peptide‐Membrane Interactions of Aib‐Rich Peptides: Multitechnique Experimental and Theoretical Analysis

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    SYSNO ASEP0542286
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleInsight into the Mechanism of Action and Peptide‐Membrane Interactions of Aib‐Rich Peptides: Multitechnique Experimental and Theoretical Analysis
    Author(s) Lizio, M. G. (GB)
    Campana, M. (GB)
    De Poli, M. (GB)
    Jefferies, D. F. (GB)
    Cullen, W. (GB)
    Andrushchenko, Valery (UOCHB-X) RID, ORCID
    Chmel, N. P. (GB)
    Bouř, Petr (UOCHB-X) RID, ORCID
    Khalid, S. (GB)
    Clayden, J. (GB)
    Blanch, E. (AU)
    Rodger, A. (GB)
    Webb, S. J. (GB)
    Source TitleChembiochem - ISSN 1439-4227
    Roč. 22, č. 9 (2021), s. 1656-1667
    Number of pages12 s.
    Languageeng - English
    CountryDE - Germany
    Keywordsantimicrobial peptides ; lipid-peptide interactions ; membranes ; molecular dynamics ; peptaibols ; transfer of chirality
    OECD categoryBiophysics
    R&D ProjectsEF16_019/0000729 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportUOCHB-X - RVO:61388963
    UT WOS000621036300001
    EID SCOPUS85101328521
    DOI10.1002/cbic.202000834
    AnnotationThe increase in resistant bacterial strains necessitates the identification of new antimicrobial molecules. Antimicrobial peptides (AMPs) are an attractive option because of evidence that bacteria cannot easily develop resistance to AMPs. The peptaibols, a class of naturally occurring AMPs, have shown particular promise as antimicrobial drugs, but their development has been hindered by their mechanism of action not being clearly understood. To explore how peptaibols might interact with membranes, circular dichroism, vibrational circular dichroism, linear dichroism, Raman spectroscopy, Raman optical activity, neutron reflectivity and molecular dynamics simulations have been used to study a small library of peptaibol mimics, the Aib‐rich peptides. All the peptides studied quickly partitioned and oriented in membranes, and we found evidence of chiral interactions between the phospholipids and membrane‐embedded peptides. The protocols presented in this paper open new ground by showing how chiro‐optical spectroscopies can throw light on the mechanism of action of AMPs.
    WorkplaceInstitute of Organic Chemistry and Biochemistry
    Contactasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
    Year of Publishing2022
    Electronic addresshttps://doi.org/10.1002/cbic.202000834
Number of the records: 1