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Insight into the Mechanism of Action and Peptide‐Membrane Interactions of Aib‐Rich Peptides: Multitechnique Experimental and Theoretical Analysis
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SYSNO ASEP 0542286 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Insight into the Mechanism of Action and Peptide‐Membrane Interactions of Aib‐Rich Peptides: Multitechnique Experimental and Theoretical Analysis Author(s) Lizio, M. G. (GB)
Campana, M. (GB)
De Poli, M. (GB)
Jefferies, D. F. (GB)
Cullen, W. (GB)
Andrushchenko, Valery (UOCHB-X) RID, ORCID
Chmel, N. P. (GB)
Bouř, Petr (UOCHB-X) RID, ORCID
Khalid, S. (GB)
Clayden, J. (GB)
Blanch, E. (AU)
Rodger, A. (GB)
Webb, S. J. (GB)Source Title Chembiochem. - : Wiley - ISSN 1439-4227
Roč. 22, č. 9 (2021), s. 1656-1667Number of pages 12 s. Language eng - English Country DE - Germany Keywords antimicrobial peptides ; lipid-peptide interactions ; membranes ; molecular dynamics ; peptaibols ; transfer of chirality OECD category Biophysics R&D Projects EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UOCHB-X - RVO:61388963 UT WOS 000621036300001 EID SCOPUS 85101328521 DOI 10.1002/cbic.202000834 Annotation The increase in resistant bacterial strains necessitates the identification of new antimicrobial molecules. Antimicrobial peptides (AMPs) are an attractive option because of evidence that bacteria cannot easily develop resistance to AMPs. The peptaibols, a class of naturally occurring AMPs, have shown particular promise as antimicrobial drugs, but their development has been hindered by their mechanism of action not being clearly understood. To explore how peptaibols might interact with membranes, circular dichroism, vibrational circular dichroism, linear dichroism, Raman spectroscopy, Raman optical activity, neutron reflectivity and molecular dynamics simulations have been used to study a small library of peptaibol mimics, the Aib‐rich peptides. All the peptides studied quickly partitioned and oriented in membranes, and we found evidence of chiral interactions between the phospholipids and membrane‐embedded peptides. The protocols presented in this paper open new ground by showing how chiro‐optical spectroscopies can throw light on the mechanism of action of AMPs. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2022 Electronic address https://doi.org/10.1002/cbic.202000834
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