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Quadruplex-Forming Motif Inserted into 3 ' UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast

SYSNO ASEP	0542265
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quadruplex-Forming Motif Inserted into 3 ' UTR of Ty1his3-AI Retrotransposon Inhibits Retrotransposition in Yeast
Author(s)	Tokan, Viktor (BFU-R)_ORCID Lorenzo, Jose Luis Rodriguez (BFU-R) Jedlička, Pavel (BFU-R)_ORCID Kejnovská, Iva (BFU-R)_{RID, ORCID} Hobza, Roman (BFU-R)_{RID, ORCID} Kejnovský, Eduard (BFU-R)_{RID, ORCID}
Number of authors	6
Article number	347
Source Title	Biology. - : MDPI Roč. 10, č. 4 (2021)
Number of pages	13 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	Ty1 LTR retrotransposon ; G-quadruplex ; retrotransposition ; N-methyl mesoporphyrin (NMM) ; Pif1 helicase ; yeast
Subject RIV	BO - Biophysics
OECD category	Other biological topics
R&D Projects	GA18-00258S GA ČR - Czech Science Foundation (CSF)
	GA21-00580S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	BFU-R - RVO:68081707
UT WOS	000642722900001
DOI	10.3390/biology10040347
Annotation	Current multiagent chemotherapy regimens have improved the cure rate in acute leukemia patients, but they are highly toxic and poorly efficient in relapsed patients. To improve the treatment approaches, new specific molecules are needed. The G-quadruplexes (G4s), which are noncanonical nucleic acid structures found in specific guanine-rich DNA or RNA, are involved in many cellular events, including control of gene expression. G4s are considered as targets for the development of anticancer agents. Heterocyclic molecules are well known to target and stabilize G4 structures. Thus, a new series of 2,9-bis[(substituted-aminomethyl)phenyl]-1,10-phenanthroline derivatives (1a-i) was designed, synthesized, and evaluated against five human myeloid leukemia cell lines (K562, KU812, MV4-11, HL60, and U937). Their ability to stabilize various oncogene promoter G4 structures (c-MYC, BCL-2, and K-RAS) as well as the telomeric G4 was also determined through the fluorescence resonance energy transfer melting assay and native mass spectrometry. In addition, the more bioactive ligands 1g-i were tested for telomerase activity in HuT78 and MV4-11 protein extracts.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2079-7737/10/4/347

Number of the records: 1