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Hypersensitivity Induced by Intrathecal Bradykinin Administration Is Enhanced by N-oleoyldopamine (OLDA) and Prevented by TRPV1 Antagonist

SYSNO ASEP	0542140
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Hypersensitivity Induced by Intrathecal Bradykinin Administration Is Enhanced by N-oleoyldopamine (OLDA) and Prevented by TRPV1 Antagonist
Author(s)	Uchytilová, Eva (FGU-C) Špicarová, Diana (FGU-C)_{RID, ORCID} Paleček, Jiří (FGU-C)_{RID, ORCID}
Article number	3712
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 7 (2021)
Number of pages	12 s.
Language	eng - English
Country	CH - Switzerland
Keywords	TRPV1 ; bradykinin ; OLDA ; spinal cord ; hyperalgesia ; allodynia
Subject RIV	FH - Neurology
OECD category	Neurosciences (including psychophysiology
R&D Projects	GA20-19136S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000638655100001
EID SCOPUS	85103479134
DOI	10.3390/ijms22073712
Annotation	Transient receptor potential vanilloid 1 (TRPV1) channels contribute to the development of several chronic pain states and represent a possible therapeutic target in many painful disease treatment. Proinflammatory mediator bradykinin (BK) sensitizes TRPV1, whereas noxious peripheral stimulation increases BK level in the spinal cord. Here, we investigated the involvement of spinal TRPV1 in thermal and mechanical hypersensitivity, evoked by intrathecal (i.t.) administration of BK and an endogenous agonist of TRPV1, N-oleoyldopamine (OLDA), using behavioral tests and i.t. catheter implantation, and administration of BK-induced transient thermal and mechanical hyperalgesia and mechanical allodynia. All these hypersensitive states were enhanced by co-administration of a low dose of OLDA (0.42 mu g i.t.), which was ineffective only under the control conditions. Intrathecal pretreatment with TRPV1 selective antagonist SB366791 prevented hypersensitivity induced by i.t. co-administration of BK and OLDA. Our results demonstrate that both thermal and mechanical hypersensitivity evoked by co-administration of BK and OLDA is mediated by the activation of spinal TRPV1 channels.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/7/3712

Number of the records: 1