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Horizontal deformation of skeletal muscle thick sections visualised by confocal microscopy
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SYSNO ASEP 0542133 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Horizontal deformation of skeletal muscle thick sections visualised by confocal microscopy Author(s) Umek, N. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Cvetko, E. (SI)
Eržen, I. (SI)Source Title Journal of Microscopy. - : Wiley - ISSN 0022-2720
Roč. 282, č. 2 (2021), s. 113-122Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords 3D ; fibre diameter ; horizontal dilation ; sample deformation ; shrinkage in Z direction Subject RIV FP - Other Medical Disciplines OECD category Anatomy and morphology (plant science to be 1.6) R&D Projects LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000594502600001 EID SCOPUS 85096685198 DOI 10.1111/jmi.12985 Annotation Certain morphological parameters of the skeletal muscle tissue can be better understood via 3D considerations. Fluorescent confocal microscopy of thick tissue sections is a well-established method for visualising and measuring skeletal muscle fibres and surrounding capillaries in 3D. However, thick tissue sections are prone to deformations which may significantly influence some stereological and morphometric results like muscle fibre diameter and capillary length, but not dimensionless parameters like object number and Euler-Poincare characteristics. To better understand this phenomenon, we studied the horizontal deformation of thick (100 mu m) transverse skeletal muscle sections, by comparing the muscle fibre diameters measured on thick sections to muscle fibre diameters measured on thin (10 mu m) sections of the same sample. Diameter changes were further correlated with shrinkage in the Z direction (axial shrinkage) and deviation of the muscle fibre preferential axis from the Z-axis. We showed that the thick sections dilated in horizontal and shrunk in Z direction, and that the magnitude of horizontal dilation was associated with the magnitude of shrinkage in the Z direction. The latter was more pronounced in transversely than obliquely cut tissue sections. The results emphasise that even when shrinkage in the Z direction can be corrected using calibration, it is important to optimise histological protocols to minimise the Z-axis collapse that could cause horizontal dilation. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1111/jmi.12985
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