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Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies
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SYSNO ASEP 0542103 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies Author(s) Ujčíková, Hana (FGU-C) RID, ORCID
Hejnová, L. (CZ)
Eckhardt, Adam (FGU-C) RID, ORCID
Roubalová, Lenka (FGU-C) RID, ORCID, SAI
Novotný, J. (CZ)
Svoboda, Petr (FGU-C) RID, ORCIDArticle number 104975 Source Title Neurochemistry International. - : Elsevier - ISSN 0197-0186
Roč. 144, Mar (2021)Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords long-term morphine withdrawal ; phosphoproteomic analysis ; nLC-MS ; label-free quantification ; 14-3-3 proteins Subject RIV ED - Physiology OECD category Physiology (including cytology) R&D Projects GA19-03295S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000636076900001 EID SCOPUS 85100027342 DOI 10.1016/j.neuint.2021.104975 Annotation Opioid addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations. However, there is a lack of information about the changes at both the cellular and molecular levels occurring after cessation of drug administration. The aim of our study was to determine alterations of both phosphoproteome and proteome in selected brain regions of the rats (brain cortex, hippocampus, striatum, and cerebellum) 3 months after cessation of 10-day morphine treatment. Phosphoproteome profiling was performed by Pro-Q® Diamond staining. The gel-based proteomic approach accompanied by label-free quantification (MaxLFQ) was used for characterization of proteome changes.The phosphoproteomic analysis revealed the largest change in the hippocampus (14), only few altered proteins were detected in the forebrain cortex (5), striatum (4), and cerebellum (3). The change of total protein composition, determined by 2D electrophoresis followed by LFQ analysis, identified 22 proteins with significantly altered expression levels in the forebrain cortex, 19 proteins in the hippocampus, 12 in the striatum and 10 in the cerebellum. The majority of altered proteins were functionally related to energy metabolism and cytoskeleton reorganization. As the most important change we regard down-regulation of 14-3-3 proteins in rat cortex and hippocampus.Our findings indicate that i) different parts of the brain respond in a distinct manner to the protracted morphine withdrawal, ii) characterize changes of protein composition in these brain parts, and iii) enlarge the scope of evidence for adaptability and distinct neuroplasticity proceeding in the brain of drug-addicted organism. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.neuint.2021.104975
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