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G-quadruplexes in H1N1 influenza genomes
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SYSNO ASEP 0541909 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title G-quadruplexes in H1N1 influenza genomes Author(s) Brázda, Václav (BFU-R) RID, ORCID
Porubiakova, Otilia (BFU-R)
Cantara, Alessio (BFU-R)
Bohalova, Natalia (BFU-R) ORCID
Coufal, Jan (BFU-R) ORCID
Bartas, Martin (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Mergny, Jean-Louis (BFU-R) ORCID, RIDNumber of authors 8 Article number 77 Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 22, č. 1 (2021)Number of pages 11 s. Publication form Online - E Language eng - English Country US - United States Keywords Influenza virus ; G-quadruplex ; G4Hunter Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000613379300001 EID SCOPUS 85099932787 DOI 10.1186/s12864-021-07377-9 Annotation BackgroundInfluenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment.ResultsPQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS, even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods.ConclusionsThe results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9
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