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Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood
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SYSNO ASEP 0541721 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood Author(s) Pastucha, M. (CZ)
Odstrčilíková, E. (CZ)
Hlaváček, Antonín (UIACH-O) ORCID
Brandmeier, J. C. (DE)
Vykoukal, V. (CZ)
Weisová, Julie (UIACH-O)
Gorris, H. H. (DE)
Skládal, P. (CZ)
Farka, Z. (CZ)Number of authors 9 Article number 6900311 Source Title IEEE Journal of Selected Topics in Quantum Electronics - ISSN 1077-260X
Roč. 27, č. 5 (2021)Number of pages 11 s. Publication form Print - P Language eng - English Country US - United States Keywords bacterial pathogens ; Paenibacillus larvae ; photon-upconversion nanoparticle ; upconversion-linked immunosorbent assay Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GJ18-03367Y GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000630043400002 EID SCOPUS 85099206204 DOI 10.1109/JSTQE.2021.3049689 Annotation American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most destructive disease of the honeybee brood. Therefore, rapid and sensitive detection methods are required to limit spreading of this pathogen, which has a major impact on agriculture and biodiversity. While P. larvae is typically detected by microbial cultivation or polymerase chain reaction, antibody-based detection represents a viable alternative. Here, we prepared an antibody specific for P. larvae and used it for the development of an upconversion-linked immunosorbent assay (ULISA). Photon-upconversion nanoparticles (UCNP) were conjugated to streptavidin via a PEG-linker using copper-catalyzed click chemistry to replace the enzyme label in conventional enzyme-linked immunosorbent assay (ELISA). The ULISA showed low cross-reactivity and provided a limit of detection of 2.9 x 10(3) CFU/mL, representing a 22-fold improvement compared to the ELISA. This level is within the bacterial loads present in honeybee larvae during an AFB infection. The assay was successfully applied to the analysis of spiked samples of bees, larvae, and hive debris. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0319251
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