Gap Junctional Communication via Connexin43 between Purkinje Fibers and Working Myocytes Explains the Epicardial Activation Pattern in the Postnatal Mouse Left Ventricle

Olejníčková, Veronika; Kočka, M.; Kvasilová, A.; Kolesová, Hana; Dziacky, A.; Gidor, T.; Gidor, L.; Šaňková, B.; Gregorovičová, Martina; Gourdie, R. G.; Sedmera, David

doi:https://dx.doi.org/10.3390/ijms22052475

Number of the records: 1

Gap Junctional Communication via Connexin43 between Purkinje Fibers and Working Myocytes Explains the Epicardial Activation Pattern in the Postnatal Mouse Left Ventricle

1.

SYSNO ASEP	0541702
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Gap Junctional Communication via Connexin43 between Purkinje Fibers and Working Myocytes Explains the Epicardial Activation Pattern in the Postnatal Mouse Left Ventricle
Author(s)	Olejníčková, Veronika (FGU-C)_{RID, ORCID, SAI} Kočka, M. (CZ) Kvasilová, A. (CZ) Kolesová, Hana (FGU-C)_{RID, ORCID, SAI} Dziacky, A. (CZ) Gidor, T. (CZ) Gidor, L. (CZ) Šaňková, B. (CZ) Gregorovičová, Martina (FGU-C)_{RID, ORCID, SAI} Gourdie, R. G. (US) Sedmera, David (FGU-C)_{RID, ORCID, SAI}
Article number	2475
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 5 (2021)
Number of pages	14 s.
Language	eng - English
Country	CH - Switzerland
Keywords	connexin ; cardiac conduction system ; optical mapping ; myocardium ; immunohistochemistry
Subject RIV	ED - Physiology
OECD category	Physiology (including cytology)
R&D Projects	GA18-03461S GA ČR - Czech Science Foundation (CSF)
	LTC17023 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000628306200001
EID SCOPUS	85101697987
DOI	10.3390/ijms22052475
Annotation	The mammalian ventricular myocardium forms a functional syncytium due to flow of electrical current mediated in part by gap junctions localized within intercalated disks. The connexin (Cx) subunit of gap junctions have direct and indirect roles in conduction of electrical impulse from the cardiac pacemaker via the cardiac conduction system (CCS) to working myocytes. Cx43 is the dominant isoform in these channels. We have studied the distribution of Cx43 junctions between the CCS and working myocytes in a transgenic mouse model, which had the His-Purkinje portion of the CCS labeled with green fluorescence protein. The highest number of such connections was found in a region about one-third of ventricular length above the apex, and it correlated with the peak proportion of Purkinje fibers (PFs) to the ventricular myocardium. At this location, on the septal surface of the left ventricle, the insulated left bundle branch split into the uninsulated network of PFs that continued to the free wall anteriorly and posteriorly. The second peak of PF abundance was present in the ventricular apex. Epicardial activation maps correspondingly placed the site of the first activation in the apical region, while some hearts presented more highly located breakthrough sites. Taken together, these results increase our understanding of the physiological pattern of ventricular activation and its morphological underpinning through detailed CCS anatomy and distribution of its gap junctional coupling to the working myocardium.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/5/2475

Number of the records: 1