Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity

Sistilli, Gabriella; Kalendová, Veronika; Čajka, Tomáš; Irodenko, Ilaria; Bardová, Kristina; Oseeva, Marina; Žáček, P.; Kroupová, Petra; Horáková, Olga; Lackner, K.; Gastaldelli, A.; Kuda, Ondřej; Kopecký, Jan; Rossmeisl, Martin

doi:https://dx.doi.org/10.3390/nu13020437

Number of the records: 1

Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity

1.

SYSNO ASEP	0541673
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity
Author(s)	Sistilli, Gabriella (FGU-C) Kalendová, Veronika (FGU-C)_{RID, ORCID} Čajka, Tomáš (FGU-C)_{RID, ORCID, SAI} Irodenko, Ilaria (FGU-C) Bardová, Kristina (FGU-C)_{RID, ORCID, SAI} Oseeva, Marina (FGU-C)_{ORCID, RID} Žáček, P. (CZ) Kroupová, Petra (FGU-C)_ORCID Horáková, Olga (FGU-C)_{RID, ORCID} Lackner, K. (AT) Gastaldelli, A. (IT) Kuda, Ondřej (FGU-C)_{RID, ORCID, SAI} Kopecký, Jan (FGU-C)_{RID, ORCID} Rossmeisl, Martin (FGU-C)_{RID, ORCID}
Article number	437
Source Title	Nutrients. - : MDPI Roč. 13, č. 2 (2021)
Number of pages	24 s.
Language	eng - English
Country	CH - Switzerland
Keywords	NAFLD ; obesity ; omega-3 ; krill oil ; phospholipids ; high-fat diet ; C57BL/6N mice ; thermoneutral temperature
Subject RIV	FB - Endocrinology, Diabetology, Metabolism, Nutrition
OECD category	Endocrinology and metabolism (including diabetes, hormones)
R&D Projects	GA17-11027S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000622893700001
EID SCOPUS	85099993828
DOI	10.3390/nu13020437
Annotation	Preclinical evidence suggests that n-3 fatty acids EPA and DHA (Omega-3) supplemented as phospholipids (PLs) may be more effective than triacylglycerols (TAGs) in reducing hepatic steatosis. To further test the ability of Omega-3 PLs to alleviate liver steatosis, we used a model of exacerbated non-alcoholic fatty liver disease based on high-fat feeding at thermoneutral temperature. Male C57BL/6N mice were fed for 24 weeks a lard-based diet given either alone (LHF) or supplemented with Omega-3 (30 mg/g diet) as PLs (krill oil, w3PL) or TAGs (Epax 3000TG concentrate, w3TG), which had a similar total content of EPA and DHA and their ratio. Substantial levels of TAG accumulation (similar to 250 mg/g) but relatively low inflammation/fibrosis levels were achieved in the livers of control LHF mice. Liver steatosis was reduced by >40% in the omega 3PL but not omega 3TG group, and plasma ALT levels were markedly reduced (by 68%) in omega 3PL mice as well. Krill oil administration also improved hepatic insulin sensitivity, and its effects were associated with high plasma adiponectin levels (150% of LHF mice) along with superior bioavailability of EPA, increased content of alkaloids stachydrine and trigonelline, suppression of lipogenic gene expression, and decreased diacylglycerol levels in the liver. This study reveals that in addition to Omega-3 PLs, other constituents of krill oil, such as alkaloids, may contribute to its strong antisteatotic effects in the liver.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2072-6643/13/2/437

Number of the records: 1