Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films

Steinerová, Marie; Matějka, Roman; Štěpanovská, Jana; Filová, Elena; Staňková, Ľubica; Rysová, M.; Martinová, L.; Dragounová, Helena; Domonkos, Mária; Artemenko, Anna; Babchenko, Oleg; Otáhal, M.; Bačáková, Lucie; Kromka, Alexander

doi:https://dx.doi.org/10.1016/j.msec.2020.111792

Number of the records: 1

Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films

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SYSNO ASEP	0541653
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Human osteoblast-like SAOS-2 cells on submicron-scale fibers coated with nanocrystalline diamond films
Author(s)	Steinerová, Marie (FGU-C)_ORCID Matějka, Roman (FGU-C)_{RID, ORCID, SAI} Štěpanovská, Jana (FGU-C)_{ORCID, RID} Filová, Elena (FGU-C)_{RID, ORCID} Staňková, Ľubica (FGU-C)_{RID, ORCID} Rysová, M. (CZ) Martinová, L. (CZ) Dragounová, Helena (FGU-C) Domonkos, Mária (FZU-D)_RID Artemenko, Anna (FZU-D)_{RID, ORCID} Babchenko, Oleg (FZU-D)_{RID, ORCID} Otáhal, M. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID} Kromka, Alexander (FZU-D)_{RID, ORCID, SAI}
Article number	111792
Source Title	Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931 Roč. 121, Feb (2021)
Number of pages	15 s.
Language	eng - English
Country	NL - Netherlands
Keywords	nanocrystalline diamond ; submicron fibers ; hierarchical organization ; silica nanostructures ; osteoblast proliferation ; osteogenic differentiation ; collagen ; alkaline phosphatase ; osteocalcin
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Biomaterials (as related to medical implants, devices, sensors)
Subject RIV - cooperation	Institute of Physics - Biophysics
R&D Projects	GA19-02891S GA ČR - Czech Science Foundation (CSF)
	NV19-02-00068 GA MZd - Ministry of Health (MZ)
	EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	CzechNanoLab - 90110 - Vysoké učení technické v Brně
Method of publishing	Limited access
Institutional support	FGU-C - RVO:67985823 ; FZU-D - RVO:68378271
UT WOS	000619124600007
EID SCOPUS	85098711683
DOI	10.1016/j.msec.2020.111792
Annotation	A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nanostructure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (HNCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated 0-SiO x samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10 degrees), while the H-NCD-coated samples were hydrophobic (contact angle >701. NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://doi.org/10.1016/j.msec.2020.111792

Number of the records: 1