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Beta-Titanium Alloy Covered by Ferroelectric Coating-Physicochemical Properties and Human Osteoblast-Like Cell Response

SYSNO ASEP	0541646
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Beta-Titanium Alloy Covered by Ferroelectric Coating-Physicochemical Properties and Human Osteoblast-Like Cell Response
Author(s)	Vandrovcová, Marta (FGU-C)_{RID, ORCID} Tolde, Z. (CZ) Vaněk, Přemysl (FZU-D)_{RID, ORCID} Nehasil, V. (CZ) Doubková, Martina (FGU-C)_{ORCID, RID} Trávníčková, Martina (FGU-C)_{RID, ORCID, SAI} Drahokoupil, Jan (FZU-D)_{RID, ORCID} Buixaderas, Elena (FZU-D)_{RID, ORCID} Borodavka, Fedir (FZU-D)_{RID, ORCID} Nováková, J. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Article number	210
Source Title	Coatings. - : MDPI Roč. 11, č. 2 (2021)
Number of pages	25 s.
Language	eng - English
Country	CH - Switzerland
Keywords	metallic bone implants ; electroactive coating ; electrical charge ; polarization ; ferroelectricity ; cell adhesion ; cell proliferation ; osteogenic differentiation ; bone tissue engineering
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Biomaterials (as related to medical implants, devices, sensors)
Subject RIV - cooperation	Institute of Physics - Biophysics
R&D Projects	GA20-01570S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; FZU-D - RVO:68378271
UT WOS	000622376700001
EID SCOPUS	85100950917
DOI	10.3390/coatings11020210
Annotation	Beta-titanium alloys are promising materials for bone implants due to their advantageous mechanical properties. For enhancing the interaction of bone cells with this perspective material, we developed a ferroelectric barium titanate (BaTiO3) coating on a Ti39Nb alloy by hydrothermal synthesis. This coating was analyzed by scanning electron and Raman microscopy, X-ray diffraction, piezoresponse force microscopy, X-ray photoelectron spectroscopy, nanoindentation, and roughness measurement. Leaching experiments in a saline solution revealed that Ba is released from the coating. A progressive decrease of Ba concentration in the material was also found after 1, 3, and 7 days of cultivation of human osteoblast-like Saos-2 cells. On day 1, the Saos-2 cells adhered on the BaTiO3 film in higher initial numbers than on the bare alloy, but they were less spread, and their initial proliferation rate was slower. These cells also contained a lower amount of beta(1)-integrins and vinculin, i.e., molecules involved in cell adhesion, and produced a lower amount of collagen I. This cell behavior was attributed to a higher surface roughness of BaTiO3 film rather than to its potential cytotoxicity, because the cell viability on this film was very high, reaching almost 99%. The amount of alkaline phosphatase, an enzyme involved in bone matrix mineralization, was similar in cells on the BaTiO3-coated and uncoated alloy, and on day 7, the cells on BaTiO3 film attained a higher final cell population density. These results indicate that after some improvements, particularly in its roughness and stability, the hydrothermal ferroelectric BaTiO3 film could be promising coating for improved osseointegration of bone implants.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2079-6412/11/2/210

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