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Regulation and activity of CaTrk1, CaAcu1 and CaHak1, the three plasma membrane potassium transporters in Candida albicans
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SYSNO ASEP 0541550 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Regulation and activity of CaTrk1, CaAcu1 and CaHak1, the three plasma membrane potassium transporters in Candida albicans Author(s) Ruiz-Castilla, F. J. (ES)
Bieber, Jan (FGU-C)
Caro, G. (ES)
Michán, C. (ES)
Sychrová, Hana (FGU-C) RID, ORCID
Ramos, J. (ES)Article number 183486 Source Title Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736
Roč. 1863, č. 1 (2021)Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords Candida albicans ; potassium transport ; Trk1 ; Acu1 ; Hak1 ; heterologous expression Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20006 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access with time embargo (01.01.2022) Institutional support FGU-C - RVO:67985823 UT WOS 000594121100029 EID SCOPUS 85092920240 DOI 10.1016/j.bbamem.2020.183486 Annotation Wild-type cells of Candida albicans, the most common human fungal pathogen, are able to grow at very low micromolar concentrations of potassium in the external milieu. One of the reasons behind that behaviour is the existence of three different types of K+ transporters in their plasma membrane: Trk1, Acu1 and Hak1. This work shows that the transporters are very differently regulated at the transcriptional level upon exposure to saline stress, pH alterations or K+ starvation. We propose that different transporters take the lead in the diverse environmental conditions, Trk1 being the “house-keeping” one, and Acu1/Hak1 dominating upon K+ limiting conditions. Heterologous expression of the genes coding for the three transporters in a Saccharomyces cerevisiae strain lacking its endogenous potassium transporters showed that all of them mediated cation transport but with very different efficiencies. Moreover, expression of the transporters in S. cerevisiae also affected other physiological characteristics such as sodium and lithium tolerance, membrane potential or intracellular pH, being, in general, CaTrk1 the most effective in keeping these parameters close to the usual wild-type physiological levels. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.bbamem.2020.183486
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