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Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats

SYSNO ASEP	0541546
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats
Author(s)	Mrózková, Petra (FGU-C)_{RID, ORCID} Špicarová, Diana (FGU-C)_{RID, ORCID} Paleček, Jiří (FGU-C)_{RID, ORCID}
Article number	991
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 3 (2021)
Number of pages	16 s.
Language	eng - English
Country	CH - Switzerland
Keywords	PAR2 ; TRPV1 ; synaptic transmission ; superficial dorsal horn ; spinal cord ; nociception ; peripheral inflammation ; thermal hyperalgesia ; inflammatory pain
Subject RIV	FH - Neurology
OECD category	Neurosciences (including psychophysiology
R&D Projects	GA18-09853S GA ČR - Czech Science Foundation (CSF)
	GA20-19136S GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000615302300001
EID SCOPUS	85099703025
DOI	10.3390/ijms22030991
Annotation	The mechanisms of inflammatory pain need to be identified in order to find new superior treatments. Protease-activated receptors 2 (PAR2) and transient receptor potential vanilloid 1 (TRPV1) are highly co-expressed in dorsal root ganglion neurons and implicated in pain development. Here, we examined the role of spinal PAR2 in hyperalgesia and the modulation of synaptic transmission in carrageenan-induced peripheral inflammation, using intrathecal (i.t.) treatment in the behavioral experiments and recordings of spontaneous, miniature and dorsal root stimulation-evoked excitatory postsynaptic currents (sEPSCs, mEPSCs and eEPSCs) in spinal cord slices. Intrathecal PAR2-activating peptide (AP) administration aggravated the carrageenan-induced thermal hyperalgesia, and this was prevented by a TRPV1 antagonist (SB 366791) and staurosporine i.t. pretreatment. Additionally, the frequency of the mEPSC and sEPSC and the amplitude of the eEPSC recorded from the superficial dorsal horn neurons were enhanced after acute PAR2 AP application, while prevented with SB 366791 or staurosporine pretreatment. PAR2 antagonist application reduced the thermal hyperalgesia and decreased the frequency of mEPSC and sEPSC and the amplitude of eEPSC. Our findings highlight the contribution of spinal PAR2 activation to carrageenan-induced hyperalgesia and the importance of dorsal horn PAR2 and TRPV1 receptor interactions in the modulation of nociceptive synaptic transmission.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/3/991

Number of the records: 1