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Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats

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    SYSNO ASEP0541546
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleSpinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats
    Author(s) Mrózková, Petra (FGU-C) RID, ORCID
    Špicarová, Diana (FGU-C) RID, ORCID
    Paleček, Jiří (FGU-C) RID, ORCID
    Article number991
    Source TitleInternational Journal of Molecular Sciences. - : MDPI
    Roč. 22, č. 3 (2021)
    Number of pages16 s.
    Languageeng - English
    CountryCH - Switzerland
    KeywordsPAR2 ; TRPV1 ; synaptic transmission ; superficial dorsal horn ; spinal cord ; nociception ; peripheral inflammation ; thermal hyperalgesia ; inflammatory pain
    Subject RIVFH - Neurology
    OECD categoryNeurosciences (including psychophysiology
    R&D ProjectsGA18-09853S GA ČR - Czech Science Foundation (CSF)
    GA20-19136S GA ČR - Czech Science Foundation (CSF)
    ED1.1.00/02.0109 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportFGU-C - RVO:67985823
    UT WOS000615302300001
    EID SCOPUS85099703025
    DOI10.3390/ijms22030991
    AnnotationThe mechanisms of inflammatory pain need to be identified in order to find new superior treatments. Protease-activated receptors 2 (PAR2) and transient receptor potential vanilloid 1 (TRPV1) are highly co-expressed in dorsal root ganglion neurons and implicated in pain development. Here, we examined the role of spinal PAR2 in hyperalgesia and the modulation of synaptic transmission in carrageenan-induced peripheral inflammation, using intrathecal (i.t.) treatment in the behavioral experiments and recordings of spontaneous, miniature and dorsal root stimulation-evoked excitatory postsynaptic currents (sEPSCs, mEPSCs and eEPSCs) in spinal cord slices. Intrathecal PAR2-activating peptide (AP) administration aggravated the carrageenan-induced thermal hyperalgesia, and this was prevented by a TRPV1 antagonist (SB 366791) and staurosporine i.t. pretreatment. Additionally, the frequency of the mEPSC and sEPSC and the amplitude of the eEPSC recorded from the superficial dorsal horn neurons were enhanced after acute PAR2 AP application, while prevented with SB 366791 or staurosporine pretreatment. PAR2 antagonist application reduced the thermal hyperalgesia and decreased the frequency of mEPSC and sEPSC and the amplitude of eEPSC. Our findings highlight the contribution of spinal PAR2 activation to carrageenan-induced hyperalgesia and the importance of dorsal horn PAR2 and TRPV1 receptor interactions in the modulation of nociceptive synaptic transmission.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2022
    Electronic addresshttps://www.mdpi.com/1422-0067/22/3/991
Number of the records: 1