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Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis
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SYSNO ASEP 0541492 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis Author(s) Sudzinová, Petra (MBU-M) ORCID
Kambová, Milada (MBU-M)
Ramaniuk, Olga (MBU-M) ORCID
Benda, Martin (MBU-M) ORCID
Šanderová, Hana (MBU-M) RID, ORCID
Krásný, Libor (MBU-M) RID, ORCIDArticle number 87 Source Title Microorganisms. - : MDPI
Roč. 9, č. 1 (2021)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords Bacillus subtilis ; transcription ; ribosomal RNA ; DNA topology Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA20-12109S GA ČR - Czech Science Foundation (CSF) Research Infrastructure C4SYS - 90055 - Mikrobiologický ústav AV ČR, v. v. i. Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000610593600001 EID SCOPUS 85099025032 DOI 10.3390/microorganisms9010087 Annotation The expression of rRNA is one of the most energetically demanding cellular processes and, as such, it must be stringently controlled. Here, we report that DNA topology, i.e., the level of DNA supercoiling, plays a role in the regulation of Bacillus subtilis sigma(A)-dependent rRNA promoters in a growth phase-dependent manner. The more negative DNA supercoiling in exponential phase stimulates transcription from rRNA promoters, and DNA relaxation in stationary phase contributes to cessation of their activity. Novobiocin treatment of B. subtilis cells relaxes DNA and decreases rRNA promoter activity despite an increase in the GTP level, a known positive regulator of B. subtilis rRNA promoters. Comparative analyses of steps during transcription initiation then reveal differences between rRNA promoters and a control promoter, Pveg, whose activity is less affected by changes in supercoiling. Additional data then show that DNA relaxation decreases transcription also from promoters dependent on alternative sigma factors sigma(B), sigma(D), sigma(E), sigma(F), and sigma(H) with the exception of sigma(N) where the trend is the opposite. To summarize, this study identifies DNA topology as a factor important (i) for the expression of rRNA in B. subtilis in response to nutrient availability in the environment, and (ii) for transcription activities of B. subtilis RNAP holoenzymes containing alternative sigma factors. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2076-2607/9/1/87
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