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Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars

SYSNO ASEP	0541299
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars
Author(s)	Lakhneko, O. (SK) Danchenko, M. (SK) Morgun, B. (UA) Kovac, A. (SK) Majerova, P. (SK) Škultéty, L'udovít (MBU-M)_{ORCID, RID}
Article number	3445
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 21, č. 10 (2020)
Number of pages	21 s.
Language	eng - English
Country	CH - Switzerland
Keywords	amylase-trypsin-inhibitors ; triticum-aestivum l. ; proteomic analysis ; celiac-disease ; Triticum aestivum L ; food quality ; cereal allergens ; discovery proteomics ; gluten ; celiac disease
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971
UT WOS	000539312100041
EID SCOPUS	85084786514
DOI	10.3390/ijms21103445
Annotation	Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2021
Electronic address	https://www.mdpi.com/1422-0067/21/10/3445

Number of the records: 1