Number of the records: 1
Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars
- 1.
SYSNO ASEP 0541299 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars Author(s) Lakhneko, O. (SK)
Danchenko, M. (SK)
Morgun, B. (UA)
Kovac, A. (SK)
Majerova, P. (SK)
Škultéty, L'udovít (MBU-M) ORCIDArticle number 3445 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 10 (2020)Number of pages 21 s. Language eng - English Country CH - Switzerland Keywords amylase-trypsin-inhibitors ; triticum-aestivum l. ; proteomic analysis ; celiac-disease ; Triticum aestivum L ; food quality ; cereal allergens ; discovery proteomics ; gluten ; celiac disease Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000539312100041 EID SCOPUS 85084786514 DOI 10.3390/ijms21103445 Annotation Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.mdpi.com/1422-0067/21/10/3445
Number of the records: 1