Number of the records: 1  

Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars

  1. 1.
    SYSNO ASEP0541299
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleComprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars
    Author(s) Lakhneko, O. (SK)
    Danchenko, M. (SK)
    Morgun, B. (UA)
    Kovac, A. (SK)
    Majerova, P. (SK)
    Škultéty, L'udovít (MBU-M) ORCID
    Article number3445
    Source TitleInternational Journal of Molecular Sciences. - : MDPI
    Roč. 21, č. 10 (2020)
    Number of pages21 s.
    Languageeng - English
    CountryCH - Switzerland
    Keywordsamylase-trypsin-inhibitors ; triticum-aestivum l. ; proteomic analysis ; celiac-disease ; Triticum aestivum L ; food quality ; cereal allergens ; discovery proteomics ; gluten ; celiac disease
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    Method of publishingOpen access
    Institutional supportMBU-M - RVO:61388971
    UT WOS000539312100041
    EID SCOPUS85084786514
    AnnotationBread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná,, Tel.: 241 062 231
    Year of Publishing2021
    Electronic address
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.