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POS-P-2170 Cathodoluminescence imaging of cell organelles and biomineralized structures observed using cryo-scanning electron microscope
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SYSNO ASEP 0541022 Document Type A - Abstract R&D Document Type O - Ostatní Title POS-P-2170 Cathodoluminescence imaging of cell organelles and biomineralized structures observed using cryo-scanning electron microscope Author(s) Vancová, Marie (BC-A) RID, ORCID
Dolejší, M. (CZ)
Vaněček, Jiří (BC-A)
Skoupý, Radim (UPT-D) RID, ORCID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Nebesářová, Jana (BC-A) RID, ORCIDNumber of authors 6 Source Title CSMS conference Microscopy 2020, online meeting 5th-6th 2020. - Praha : Československá mikroskopická společnost, z.s., 2020
S. 57-57Number of pages 1 s. Publication form Online - E Action CSMS conference Microscopy 2020, online meeting 6th-7th 2020 Event date 06.10.2021 - 07.10.2021 VEvent location Lednice Country CZ - Czech Republic Event type CST Language eng - English Country CZ - Czech Republic Keywords Nanomechanical ; Lyme disease ; extracellular matrix Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Scientific Instruments - Electronics ; Optoelectronics, Electrical Engineering R&D Projects 8J19AT009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-21244S GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 ; UPT-D - RVO:68081731 Annotation As opposed to pathogens passively circulating in the body fluids of their host, pathogenic species within the Spirochaetes phylum are able to actively coordinate their movement in the host to cause systemic infections. Based on the unique morphology and high motility of spirochetes, we hypothesized that their surface adhesive molecules might be suitably adapted to aid in their dissemination strategies. Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly DbpA/B, can strongly enhance motility of spirochetes in the extracellular matrix of the host. Using atomic force microscopy, we disentangled the mechanistic details of DbpA/B and decorin/laminin interactions. Our results show that spirochetes are able to leverage a wide variety of adhesion strategies through force-tuning transient molecular binding to extracellular matrix components, in order to switch between different motility states to enhance its dissemination Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address http://microscopy.cz/conferencecsms/csms-sbornik.pdf
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