Protein downstream processing: Design, Development And Application of High and Low-Resolution Methods (N. Labrou Ed.) 2nd edition

Dráber, Pavel; Sulimenko, Tetyana; Sulimenko, Vadym; Dráberová, Eduarda

doi:https://dx.doi.org/10.1007/978-1-0716-0775-6_27

Number of the records: 1

Protein downstream processing: Design, Development And Application of High and Low-Resolution Methods (N. Labrou Ed.) 2nd edition

1.

SYSNO ASEP	0540021
Document Type	M - Monograph Chapter
R&D Document Type	Monograph Chapter
Title	Stabilization of proteins by freeze-drying in the presence of trehalose: a case study of tubulin
Author(s)	Dráber, Pavel (UMG-J)_{RID, ORCID} Sulimenko, Tetyana (UMG-J) Sulimenko, Vadym (UMG-J)_{RID, ORCID} Dráberová, Eduarda (UMG-J)_{RID, ORCID}
Source Title	Protein downstream processing: Design, Development And Application of High and Low-Resolution Methods (N. Labrou Ed.) 2nd edition, Stabilization of proteins by freeze-drying in the presence of trehalose: a case study of tubulin. - New York : Humana Press, 2021 - ISBN 978-1-0716-0775-6
Pages	s. 417-435
Number of pages	19 s.
Number of pages	511
Publication form	Online - E
Language	eng - English
Country	US - United States
Keywords	freeze-drying ; trehalose ; tubulin
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Biochemistry and molecular biology
R&D Projects	GA18-27197S GA ČR - Czech Science Foundation (CSF)
	GA19-20716S GA ČR - Czech Science Foundation (CSF)
	LTAUSA17052 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMG-J - RVO:68378050
UT WOS	000697782600028
DOI	10.1007/978-1-0716-0775-6_27
Annotation	Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into a nonpolymerizing state. For this reason, it is usually stored at -80 C or liquid nitrogen to preserve its conformation and polymerization properties. In this chapter, we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose, and methods enabling the evaluation of tubulin functions in rehydrated samples.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2023
Electronic address	https://link.springer.com/protocol/10.1007%2F978-1-0716-0775-6_27

Number of the records: 1