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Suicidal Leishmania
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SYSNO ASEP 0540011 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Suicidal Leishmania Author(s) Podešvová, L. (CZ)
Leštinová, T. (CZ)
Horáková, Eva (BC-A) RID, ORCID
Lukeš, Julius (BC-A) RID, ORCID
Volf, P. (CZ)
Yurchenko, V. (CZ)Number of authors 6 Article number 79 Source Title Pathogens. - : MDPI
Roč. 9, č. 2 (2020)Number of pages 12 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords phospholipase a(2) ; snake-venom ; antimicrobial peptides ; protective immunity ; expression ; vaccine ; myotoxins ; system ; toxin ; Leishmania mexicana ; suicidal system ; ecDHFR ; BnSP-7 ; apoptosis Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LL1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000519242900073 EID SCOPUS 85078732155 DOI 10.3390/pathogens9020079 Annotation Leishmania are obligate intracellular parasites known to have developed successful ways of efficient immunity evasion. Because of this, leishmaniasis, a disease caused by these flagellated protists, is ranked as one of the most serious tropical infections worldwide. Neither prophylactic medication, nor vaccination has been developed thus far, even though the infection has usually led to strong and long-lasting immunity. In this paper, we describe a “suicidal” system established in Leishmania mexicana, a human pathogen causing cutaneous leishmaniasis. This system is based on the expression and (de)stabilization of a basic phospholipase A2 toxin from the Bothrops pauloensis snake venom, which leads to the inducible cell death of the parasites in vitro. Furthermore, the suicidal strain was highly attenuated during macrophage infection, regardless of the toxin stabilization. Such a deliberately weakened parasite could be used to vaccinate the host, as its viability is regulated by the toxin stabilization, causing a profoundly reduced pathogenesis. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://www.mdpi.com/2076-0817/9/2/79
Number of the records: 1