Number of the records: 1  

Suicidal Leishmania

    1.
    SYSNO ASEP0540011
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleSuicidal Leishmania
    Author(s) Podešvová, L. (CZ)
    Leštinová, T. (CZ)
    Horáková, Eva (BC-A) RID, ORCID
    Lukeš, Julius (BC-A) RID, ORCID
    Volf, P. (CZ)
    Yurchenko, V. (CZ)
    Number of authors6
    Article number79
    Source TitlePathogens. - : MDPI
    Roč. 9, č. 2 (2020)
    Number of pages12 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordsphospholipase a(2) ; snake-venom ; antimicrobial peptides ; protective immunity ; expression ; vaccine ; myotoxins ; system ; toxin ; Leishmania mexicana ; suicidal system ; ecDHFR ; BnSP-7 ; apoptosis
    Subject RIVEB - Genetics ; Molecular Biology
    OECD categoryGenetics and heredity (medical genetics to be 3)
    R&D ProjectsLL1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportBC-A - RVO:60077344
    UT WOS000519242900073
    EID SCOPUS85078732155
    DOI10.3390/pathogens9020079
    AnnotationLeishmania are obligate intracellular parasites known to have developed successful ways of efficient immunity evasion. Because of this, leishmaniasis, a disease caused by these flagellated protists, is ranked as one of the most serious tropical infections worldwide. Neither prophylactic medication, nor vaccination has been developed thus far, even though the infection has usually led to strong and long-lasting immunity. In this paper, we describe a “suicidal” system established in Leishmania mexicana, a human pathogen causing cutaneous leishmaniasis. This system is based on the expression and (de)stabilization of a basic phospholipase A2 toxin from the Bothrops pauloensis snake venom, which leads to the inducible cell death of the parasites in vitro. Furthermore, the suicidal strain was highly attenuated during macrophage infection, regardless of the toxin stabilization. Such a deliberately weakened parasite could be used to vaccinate the host, as its viability is regulated by the toxin stabilization, causing a profoundly reduced pathogenesis.
    WorkplaceBiology Centre (since 2006)
    ContactDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Year of Publishing2021
    Electronic addresshttps://www.mdpi.com/2076-0817/9/2/79
Number of the records: 1  

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