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Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors
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SYSNO ASEP 0539970 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Endothelialization of an ePTFE vessel prosthesis modified with an antithrombogenic fibrin/heparin coating enriched with bound growth factors Author(s) Táborská, Johanka (UMCH-V)
Riedelová, Zuzana (UMCH-V) ORCID
Brynda, Eduard (UMCH-V) RID
Májek, P. (CZ)
Riedel, Tomáš (UMCH-V) RID, ORCIDSource Title RSC Advances. - : Royal Society of Chemistry
Roč. 11, č. 11 (2021), s. 5903-5913Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords fibrin coating ; ePTFE vascular graft ; vascular endothelial growth factor Subject RIV EI - Biotechnology ; Bionics OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects GA18-01163S GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMCH-V - RVO:61389013 UT WOS 000614616900006 EID SCOPUS 85100483467 DOI 10.1039/D1RA00053E Annotation Early and late thrombosis remain the most frequent reasons for the failure of synthetic cardiovascular grafts. Long-term hemocompatibility of implanted synthetic grafts can be achieved if a natural living endothelium is formed over its blood-contacting surface. Here we present a modification of a standard expanded polytetrafluorethylene (ePTFE) vessel prosthesis by a controlled preparation of a fibrin mesh enriched with covalently bound heparin and noncovalently bound vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). Compared to a bare prosthesis, the coated prosthesis showed excellent antithrombogenic properties after contact with heparinized fresh human blood. Human umbilical vein endothelial cells seeded on the inner surface of the coated prosthesis formed a confluent layer in 5 days, whereas only small colonies of cells were scattered on the bare prosthesis. Viability of the cells was promoted mainly by FGF immobilized on the coating. These findings suggest that the coating may prevent acute thrombus formation and support the self-endothelialization of an implanted ePTFE vascular graft in vivo. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2022 Electronic address https://pubs.rsc.org/en/content/articlelanding/2021/RA/D1RA00053E#!divAbstract
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