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Characterization of sponge-associated Verrucomicrobia: microcompartment-based sugar utilization and enhanced toxin-antitoxin modules as features of host-associated Opitutales
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SYSNO ASEP 0538548 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of sponge-associated Verrucomicrobia: microcompartment-based sugar utilization and enhanced toxin-antitoxin modules as features of host-associated Opitutales Author(s) Sizikov, S. (IL)
Burgsdorf, I. (IL)
Handley, K.M. (NZ)
Lahyani, M. (IL)
Haber, Markus (BC-A) RID
Steindler, L. (IL)Number of authors 6 Source Title Environmental Microbiology. - : Wiley - ISSN 1462-2912
Roč. 22, č. 11 (2020), s. 4669-4688Number of pages 20 s. Language eng - English Country GB - United Kingdom Keywords phylum verrucomicrobia ; escherichia-coli ; single-cell ; sulfated polysaccharides ; l-fucose ; aggregation factor ; marine bacterium ; structural basis Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000568333700001 EID SCOPUS 85090777515 DOI 10.1111/1462-2920.15210 Annotation Bacteria of the phylumVerrucomicrobiaare ubiquitous in marine environments and can be found as free-living organisms or as symbionts of eukaryotic hosts. Little is known about host-associatedVerrucomicrobiain the marine environment. Here we reconstructed two genomes of symbioticVerrucomicrobiafrom bacterial metagenomes derived from the Atlanto-Mediterranean spongePetrosia ficiformisand three genomes from strains that we isolated from offshore seawater of the Eastern Mediterranean Sea. Phylogenomic analysis of these five strains indicated that they are all members ofVerrucomicrobiasubdivision 4, orderOpitutales. We compared these novel sponge-associated and seawater-isolated genomes to closely relatedVerrucomicrobia. Genomic analysis revealed thatPlanctomycetes-Verrucomicrobiamicrocompartment gene clusters are enriched in the genomes of symbioticOpitutalesincluding sponge symbionts but not in free-living ones. We hypothesize that in sponge symbionts these microcompartments are used for degradation ofl-fucose andl-rhamnose, which are components of algal and bacterial cell walls and therefore may be found at high concentrations in the sponge tissue. Furthermore, we observed an enrichment of toxin-antitoxin modules in symbioticOpitutales. We suggest that, in sponges, verrucomicrobial symbionts utilize these modules as a defence mechanism against antimicrobial activity deriving from the abundant microbial community co-inhabiting the host. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://doi.org/10.1111/1462-2920.15210
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