Number of the records: 1
Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface
- 1.
SYSNO ASEP 0538344 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 tat on hydrogen-terminated surface Author(s) Ahmad, N.A. (MY)
Rahim, R.A. (MY)
Rezek, Bohuslav (FZU-D) RID, ORCID
Kromka, Alexander (FZU-D) RID, ORCID, SAI
Ismail, N.S. (MY)
Gopinath, S.Ch.B. (MY)
Ižák, Tibor (FZU-D) RID
Procházka, V. (CZ)
Faudzi, F.N.M. (MY)
Abidin, A.S.Z. (MY)
Maidizn, N.N.M. (MY)Number of authors 11 Source Title International Journal of Nanoelectronics and Materials. - : Universiti Malaysia Perlis - ISSN 1985-5761
Roč. 13, č. 2 (2020), s. 295-306Number of pages 12 s. Language eng - English Country MY - Malaysia Keywords aptamer ; electrolyte-gated field effect transistor ; HIV-1 Tat ; nanocrystalline diamonds Subject RIV JB - Sensors, Measurment, Regulation OECD category Nano-materials (production and properties) R&D Projects EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support FZU-D - RVO:68378271 UT WOS 000531045800006 EID SCOPUS 85085315022 Annotation This research investigated on Trans-activator of transcription (Tat) protein, a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2021 Electronic address https://ijneam.unimap.edu.my/images/PDF/IJNEAM%20APR%202020/Vol_13_No_2_2020_7_295-306.pdf
Number of the records: 1