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Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model

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    SYSNO ASEP0537796
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
    Author(s) Vašíček, Ondřej (BFU-R) ORCID
    Hájek, Jan (MBU-M) ORCID
    Blahová, L. (CZ)
    Hrouzek, Pavel (MBU-M) ORCID
    Babica, Pavel (BU-J) RID, ORCID
    Kubala, Lukáš (BFU-R) RID, ORCID
    Šindlerová, Lenka (BFU-R) RID, ORCID
    Number of authors7
    Article number101849
    Source TitleHarmful Algae. - : Elsevier - ISSN 1568-9883
    Roč. 96, Jun 2020 (2020)
    Number of pages8 s.
    Publication formOnline - E
    Languageeng - English
    CountryUS - United States
    Keywordscell ; peptides ; proliferation ; stabilization ; toxicity ; claudins ; health
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    Subject RIV - cooperationInstitute of Microbiology - Hydrology ; Limnology
    Institute of Botany - Microbiology, Virology
    R&D ProjectsGA16-24949S GA ČR - Czech Science Foundation (CSF)
    LO1416 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportBFU-R - RVO:68081707 ; MBU-M - RVO:61388971 ; BU-J - RVO:67985939
    UT WOS000541912700014
    EID SCOPUS85086601564
    DOI10.1016/j.hal.2020.101849
    AnnotationPuwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2021
    Electronic addresshttps://doi.org/10.1016/j.hal.2020.101849
Number of the records: 1