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Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model

SYSNO ASEP	0537796
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
Author(s)	Vašíček, Ondřej (BFU-R)_{ORCID, RID} Hájek, Jan (MBU-M)_ORCID Blahová, L. (CZ) Hrouzek, Pavel (MBU-M)_ORCID Babica, Pavel (BU-J)_{RID, ORCID} Kubala, Lukáš (BFU-R)_{RID, ORCID} Šindlerová, Lenka (BFU-R)_{RID, ORCID}
Number of authors	7
Article number	101849
Source Title	Harmful Algae. - : Elsevier - ISSN 1568-9883 Roč. 96, Jun 2020 (2020)
Number of pages	8 s.
Publication form	Online - E
Language	eng - English
Country	US - United States
Keywords	cell ; peptides ; proliferation ; stabilization ; toxicity ; claudins ; health
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
Subject RIV - cooperation	Institute of Microbiology - Hydrology ; Limnology Institute of Botany - Microbiology, Virology
R&D Projects	GA16-24949S GA ČR - Czech Science Foundation (CSF)
	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	BFU-R - RVO:68081707 ; MBU-M - RVO:61388971 ; BU-J - RVO:67985939
UT WOS	000541912700014
EID SCOPUS	85086601564
DOI	10.1016/j.hal.2020.101849
Annotation	Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2021
Electronic address	https://doi.org/10.1016/j.hal.2020.101849

Number of the records: 1