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Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
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SYSNO ASEP 0537796 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model Author(s) Vašíček, Ondřej (BFU-R) ORCID, RID
Hájek, Jan (MBU-M) ORCID
Blahová, L. (CZ)
Hrouzek, Pavel (MBU-M) ORCID
Babica, Pavel (BU-J) RID, ORCID
Kubala, Lukáš (BFU-R) RID, ORCID
Šindlerová, Lenka (BFU-R) RID, ORCIDNumber of authors 7 Article number 101849 Source Title Harmful Algae. - : Elsevier - ISSN 1568-9883
Roč. 96, Jun 2020 (2020)Number of pages 8 s. Publication form Online - E Language eng - English Country US - United States Keywords cell ; peptides ; proliferation ; stabilization ; toxicity ; claudins ; health Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Microbiology - Hydrology ; Limnology
Institute of Botany - Microbiology, VirologyR&D Projects GA16-24949S GA ČR - Czech Science Foundation (CSF) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 ; MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000541912700014 EID SCOPUS 85086601564 DOI 10.1016/j.hal.2020.101849 Annotation Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2021 Electronic address https://doi.org/10.1016/j.hal.2020.101849
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