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Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography

SYSNO ASEP	0537739
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography
Author(s)	Shapoval, Oleksandr (UMCH-V)_{ORCID, RID} Oleksa, Viktoriia (UMCH-V)_RID Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Lobaz, Volodymyr (UMCH-V)_{RID, ORCID} Trhlíková, Olga (UMCH-V)_{RID, ORCID} Filipová, Marcela (UMCH-V)_{RID, ORCID} Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID} Engstová, Hana (FGU-C)_{RID, ORCID} Pankrác, J. (CZ) Modrý, A. (CZ) Herynek, V. (CZ) Ježek, Petr (FGU-C)_{RID, ORCID} Šefc, L. (CZ) Horák, Daniel (UMCH-V)_{RID, ORCID}
Article number	230
Source Title	Nanomaterials. - : MDPI Roč. 11, č. 1 (2021)
Number of pages	21 s.
Language	eng - English
Country	CH - Switzerland
Keywords	up-conversion luminescence ; down-conversion luminescence ; colloidal stability
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
Subject RIV - cooperation	Institute of Physiology - Cell Biology
R&D Projects	GA19-00676S GA ČR - Czech Science Foundation (CSF)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC19032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Open access
Institutional support	UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823
UT WOS	000610663900001
EID SCOPUS	85100140596
DOI	10.3390/nano11010230
Annotation	Multimodal imaging, integrating several modalities including down- and up-conversion luminescence, T1- and T2(T2)-weighted MRI, and CT contrasting in one system, is very promising for improved diagnosis of severe medical disorders. To reach the goal, it is necessary to develop suitable nanoparticles that are highly colloidally stable in biologically relevant media. Here, hydrophilic poly(N,N-dimethylacrylamide-N-acryloylglycine methyl ester)-alendronate-[P(DMA-AGME)-Ale]-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles were synthesized by a coprecipitation method in ethylene glycol (EG) followed by coating with the polymer. The particles were tho-roughly characterized by a dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDAX), selected area electron diffraction (SAED), elemental ana-lysis and fluorescence spectroscopy. Aqueous particle dispersions exhibited excellent colloidal stability in water and physiological buffers. In vitro toxicity assessments suggested no or only mild toxicity of the surface-engineered Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ particles in a wide range of concentrations. Internalization of the particles by several types of cells, including HeLa, HF, HepG2, and INS, was confirmed by a down- and up-conversion confocal microscopy. Newly developed particles thus proved to be an efficient contrast agent for fluorescence imaging, T1- and T2(T2)-weighted magnetic resonance imaging (MRI), and computed tomography (CT).
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2079-4991/11/1/230

Number of the records: 1