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Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system
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SYSNO ASEP 0537554 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system Author(s) Rodriguez Lorenzo, José Luis (BFU-R)
Hubinský, Marcel (BFU-R)
Vyskot, Boris (BFU-R) RID, ORCID
Hobza, Roman (BFU-R) RID, ORCIDNumber of authors 4 Article number 110528 Source Title Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 299, č. 2020 (2020)Number of pages 7 s. Publication form Online - E Language eng - English Country IE - Ireland Keywords sex-chromosomes ; dna methylation ; dioecious plant ; recombination suppression ; repetitive sequences ; evolution ; degeneration Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects GA18-06147S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000569805800011 EID SCOPUS 85089176013 DOI 10.1016/j.plantsci.2020.110528 Annotation Silene latifolia is a model organism to study evolutionary young heteromorphic sex chromosome evolution in plants. Previous research indicates a Y-allele gene degeneration and a dosage compensation system already operating. Here, we propose an epigenetic approach based on analysis of several histone post-translational modifications (PTMs) to find the first epigenetic hints of the X:Y sex chromosome system regulation in S. latifolia. Through chromatin immunoprecipitation we interrogated six genes from X and Y alleles. Several histone PTMS linked to DNA methylation and transcriptional repression (H3K27me3, H3K23me, H3K9me2 and H3K9me3) and to transcriptional activation (H3K4me3 and H4K5, 8, 12, 16ac) were used. DNA enrichment (Immunoprecipitated DNA/input DNA) was analyzed and showed three main results: (i) promoters of the Y allele are associated with heterochromatin marks, (ii) promoters of the X allele in males are associated with activation of transcription marks and finally, (iii) promoters of X alleles in females are associated with active and repressive marks. Our finding indicates a transcription activation of X allele and transcription repression of Y allele in males. In females we found a possible differential regulation (up X-1, down X-2) of each female X allele. These results agree with the mammal-like epigenetic dosage compensation regulation. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2021 Electronic address https://www.sciencedirect.com/science/article/pii/S0168945220301333?via%3Dihub
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