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Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties
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SYSNO ASEP 0536963 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties Author(s) Nováková, Michaela (MBU-M)
Fábryová, Tereza (MBU-M) ORCID
Vokurková, D. (CZ)
Dolečková, I. (CZ)
Kopecký, Jiří (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Tůmová, L. (CZ)
Cheel, José (MBU-M) RID, ORCIDArticle number 73 Source Title Separations. - : MDPI
Roč. 7, č. 4 (2020)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords Centrifugal partition chromatography (CPC) ; Countercurrent chromatography (CCC) ; Cyanobacteria ; High-performance countercurrent chromatography (HPCCC) ; Myxoxanthophyll Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000601724400001 EID SCOPUS 85097942310 DOI https://doi.org/10.3390/separations7040073 Annotation Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.mdpi.com/2297-8739/7/4/73
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