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Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties

SYSNO ASEP	0536963
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties
Author(s)	Nováková, Michaela (MBU-M) Fábryová, Tereza (MBU-M)_ORCID Vokurková, D. (CZ) Dolečková, I. (CZ) Kopecký, Jiří (MBU-M)_ORCID Hrouzek, Pavel (MBU-M)_ORCID Tůmová, L. (CZ) Cheel, José (MBU-M)_{RID, ORCID}
Article number	73
Source Title	Separations. - : MDPI Roč. 7, č. 4 (2020)
Number of pages	15 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Centrifugal partition chromatography (CPC) ; Countercurrent chromatography (CCC) ; Cyanobacteria ; High-performance countercurrent chromatography (HPCCC) ; Myxoxanthophyll
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
R&D Projects	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971
UT WOS	000601724400001
EID SCOPUS	85097942310
DOI	10.3390/separations7040073
Annotation	Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2021
Electronic address	https://www.mdpi.com/2297-8739/7/4/73

Number of the records: 1