Number of the records: 1
Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis
- 1.
SYSNO ASEP 0536518 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis Author(s) Gotvaldová, K. (CZ)
Hájková, K. (CZ)
Borovička, Jan (UJF-V) RID, ORCID, SAI
Jurok, R. (CZ)
Cihlářová, P. (CZ)
Kuchař, M. (CZ)Number of authors 6 Source Title Drug Testing and Analysis. - : Wiley - ISSN 1942-7603
Roč. 13, č. 2 (2021), s. 439-446Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords LC-MS ; mushrooms ; psilocybin ; stability ; tryptamines Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Limited access Institutional support UJF-V - RVO:61389005 UT WOS 000585171500001 EID SCOPUS 85096679307 DOI 10.1002/dta.2950 Annotation Psilocybin, psilocin, baeocystin, norbaeocystin, and aeruginascin are tryptamines structurally similar to the neurotransmitter serotonin. Psilocybin and its pharmacologically active metabolite psilocin in particular are known for their psychoactive effects. These substances typically occur in most species of the genus Psilocybe (Fungi, Strophariaceae). Even the sclerotia of some of these fungi known as 'magic truffles' are of growing interest in microdosing due to them improving cognitive function studies. In addition to microdosing studies, psilocybin has also been applied in clinical studies, but only its pure form has been administrated so far. Moreover, the determination of tryptamine alkaloids is used in forensic analysis.
In this study, freshly cultivated fruit bodies of Psilocybe cubensis were used for monitoring stability (including storage and processing conditions of fruiting bodies). Furthermore, mycelium and the individual parts of the fruiting bodies (caps, stipes, and basidiospores) were also examined. The concentration of tryptamines in final extracts was analyzed using ultra-high-performance liquid chromatography coupled with mass spectrometry. No tryptamines were detected in the basidiospores, and only psilocin was present at 0.47 wt.% in the mycelium. The stipes contained approximately half the amount of tryptamine alkaloids (0.52 wt.%) than the caps (1.03 wt.%). However, these results were not statistically significant, as the concentration of tryptamines in individual fruiting bodies is highly variable. The storage conditions showed that the highest degradation of tryptamines was seen in fresh mushrooms stored at -80 degrees C, and the lowest decay was seen in dried biomass stored in the dark at room temperature.Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2022 Electronic address https://doi.org/10.1002/dta.2950
Number of the records: 1