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Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease
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SYSNO ASEP 0536067 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease Author(s) Tomášová, Petra (MBU-M)
Čermáková, Martina (MBU-M) ORCID
Pelantová, Helena (MBU-M) ORCID, RID
Vecka, M. (CZ)
Kratochvílová, H. (CZ)
Lipš, M. (CZ)
Lindner, J. (CZ)
Ivák, P. (CZ)
Netuka, I. (CZ)
Šedivá, Blanka (MBU-M) ORCID
Haluzník, M. (CZ)
Kuzma, Marek (MBU-M) ORCID, RIDSource Title Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893
Roč. 19, č. 10 (2020), s. 3993-4003Number of pages 11 s. Language eng - English Country US - United States Keywords epicardial adipose tissue ; subcutaneous adipose tissue ; coronary artery disease Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000577157000011 EID SCOPUS 85092681739 DOI 10.1021/acs.jproteome.0c00269 Annotation Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We performed an MS-based lipidomic analysis of subcutaneous and epicardial adipose tissue in 23 patients with coronary artery disease stratified for the presence/absence of type 2 diabetes mellitus and a control group of 13 subjects aiming at identification of factors from epicardial fat contributing to the development of coronary artery disease. The samples of adipose tissues were obtained during elective cardiac surgery. They were extracted and analyzed with and without previous triacylglycerols separation by high-pressure liquid chromatography-mass spectrometry (HPLC-MS). Multi-variate and univariate analyses were performed. Lipidomics data were correlated with biochemical parameters. We identified multiple changes in monoacylglycerols, diacylglycerols, triacylglycerols, glycerophosphatidylserines, glycerophosphatidylethanolamines, glycerophosphatidylcholines, ceramides, sphingomyelins, and derivatives of cholesterol. Observed changes included molecules with fatty acids with odd (15:0, 15:1, 17:0, 17:1) and even (10:0, 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:4, 20:1, 22:0) fatty acids in both types of adipose tissue. More pronounced changes were detected in epicardial adipose tissue compared to subcutaneous adipose tissue of patients with coronary artery disease and type 2 diabetes. Lipidomic analysis of subcutaneous and epicardial adipose tissue revealed different profiles for patients with coronary artery disease and type 2 diabetes, which might be related to coronary artery disease and the presence of type 2 diabetes. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00269
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