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Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease

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    SYSNO ASEP0536067
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleLipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease
    Author(s) Tomášová, Petra (MBU-M)
    Čermáková, Martina (MBU-M) ORCID
    Pelantová, Helena (MBU-M) ORCID, RID
    Vecka, M. (CZ)
    Kratochvílová, H. (CZ)
    Lipš, M. (CZ)
    Lindner, J. (CZ)
    Ivák, P. (CZ)
    Netuka, I. (CZ)
    Šedivá, Blanka (MBU-M) ORCID
    Haluzník, M. (CZ)
    Kuzma, Marek (MBU-M) ORCID, RID
    Source TitleJournal of Proteome Research. - : American Chemical Society - ISSN 1535-3893
    Roč. 19, č. 10 (2020), s. 3993-4003
    Number of pages11 s.
    Languageeng - English
    CountryUS - United States
    Keywordsepicardial adipose tissue ; subcutaneous adipose tissue ; coronary artery disease
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    R&D ProjectsLO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportMBU-M - RVO:61388971
    UT WOS000577157000011
    EID SCOPUS85092681739
    DOI10.1021/acs.jproteome.0c00269
    AnnotationCoronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We performed an MS-based lipidomic analysis of subcutaneous and epicardial adipose tissue in 23 patients with coronary artery disease stratified for the presence/absence of type 2 diabetes mellitus and a control group of 13 subjects aiming at identification of factors from epicardial fat contributing to the development of coronary artery disease. The samples of adipose tissues were obtained during elective cardiac surgery. They were extracted and analyzed with and without previous triacylglycerols separation by high-pressure liquid chromatography-mass spectrometry (HPLC-MS). Multi-variate and univariate analyses were performed. Lipidomics data were correlated with biochemical parameters. We identified multiple changes in monoacylglycerols, diacylglycerols, triacylglycerols, glycerophosphatidylserines, glycerophosphatidylethanolamines, glycerophosphatidylcholines, ceramides, sphingomyelins, and derivatives of cholesterol. Observed changes included molecules with fatty acids with odd (15:0, 15:1, 17:0, 17:1) and even (10:0, 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:4, 20:1, 22:0) fatty acids in both types of adipose tissue. More pronounced changes were detected in epicardial adipose tissue compared to subcutaneous adipose tissue of patients with coronary artery disease and type 2 diabetes. Lipidomic analysis of subcutaneous and epicardial adipose tissue revealed different profiles for patients with coronary artery disease and type 2 diabetes, which might be related to coronary artery disease and the presence of type 2 diabetes.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2021
    Electronic addresshttps://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00269
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