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Three-Dimensional Printed Target Plates for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
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SYSNO ASEP 0535974 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Three-Dimensional Printed Target Plates for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Author(s) Fialova, J. (CZ)
Hrabák, J. (CZ)
Studentová, V. (CZ)
Kavan, D. (CZ)
Pompach, Petr (BTO-N)
Novák, P. (CZ)Number of authors 6 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 92, č. 19 (2020), s. 12783-12788Number of pages 6 s. Language eng - English Country US - United States Keywords maldi-tof ms Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000580426800008 EID SCOPUS 85095970297 DOI 10.1021/acs.analchem.0c01737 Annotation Three-dimensional printing (3D printing) is a fast-growing technology with high impact in industry, medicine, and the life sciences. Fused deposition modeling (FDM), which uses plastic filaments extruded through a heated nozzle, is the most common 3D printing technology for creation of objects. In this work, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) target plates printed by FDM technology using conductive plastic material were evaluated for their detection capability of proteins and peptides. The 3D printed MALDI targets were validated by analysis of different types of bacteria and compared with commercially available MBT BioTargets. The results indicate that 3D printed MALDI targets are comparable to standard MBT BioTargets and stainless-steel targets and may be used for different MALDI-TOF MS applications. The 3D printing allows easy manufacturing of MALDI targets with different dimensions and spot geometry. Moreover, the 3D printed MALDI targets are disposable, cheap, and easy to produce. These features make them a suitable cost-effective alternative to conventional targets for any MALDI MS analysis. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2021 Electronic address https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01737
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