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Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach
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SYSNO ASEP 0535755 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach Author(s) Pavlíková, N. (CZ)
Šrámek, J. (CZ)
Jelínek, M. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Kovář, J. (CZ)Article number e0229430 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 15, č. 10 (2020)Number of pages 16 s. Language eng - English Country US - United States Keywords persistent organic pollutants ; organochlorine pesticides ; er stress OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000588370000016 EID SCOPUS 85094863054 DOI 10.1371/journal.pone.0229430 Annotation Many compounds have the potential to harm pancreatic beta-cells, organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p'-DDT exposure among proteins expressed in NES2Y human pancreatic beta-cells employing 2-D electrophoresis. We exposed NES2Y cells to a high concentration (150 mu M, LC96 after 72 hours) of p,p'-DDT for 24 and 30 hours and determined proteins with changed expression using 2-D electrophoresis. We have found 22 proteins that changed their expression. They included proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins involved in the maintenance of the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). The proteins we have identified may serve as indicators of p,p'-DDT toxicity in beta-cells in future studies, including long-term exposure to environmentally relevant concentrations. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229430
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