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Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

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    SYSNO ASEP0535590
    Document TypeC - Proceedings Paper (int. conf.)
    R&D Document TypeConference Paper
    TitleSurface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
    Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
    Ježek, Jan (UPT-D) RID, ORCID, SAI
    Kizovský, Martin (UPT-D)
    Klementová, Tereza (UPT-D) ORCID, SAI
    Krátký, Stanislav (UPT-D) RID, ORCID, SAI
    Sobota, Jaroslav (UPT-D) RID, ORCID, SAI
    Samek, Ota (UPT-D) RID, ORCID, SAI
    Zemánek, Pavel (UPT-D) RID, SAI, ORCID
    Buryška, T. (CZ)
    Damborský, J. (CZ)
    Prokop, Z. (CZ)
    Number of authors11
    Source TitlePhotonIcs and Electromagnetics Research Symposium - Spring, PIERS-Spring 2019. Proceedings. - New York : IEEE, 2019 - ISSN 1559-9450 - ISBN 978-1-7281-3403-1
    Pagess. 1301-1309
    Number of pages9 s.
    Publication formPrint - P
    ActionPhotonIcs and Electromagnetics Research Symposium - Spring (PIERS-Spring) 2019
    Event date17.06.2019 - 20.06.2019
    VEvent locationRome
    CountryIT - Italy
    Event typeWRD
    Languageeng - English
    CountryUS - United States
    Keywordschemical analysis ; Raman spectroscopy ; emission spectroscopy ; light transmission
    Subject RIVBH - Optics, Masers, Lasers
    OECD categoryOptics (including laser optics and quantum optics)
    R&D ProjectsGA16-07965S GA ČR - Czech Science Foundation (CSF)
    LO1212 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    ED0017/01/01 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUPT-D - RVO:68081731
    UT WOS000550769301050
    EID SCOPUS85082018253
    AnnotationOptofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
    WorkplaceInstitute of Scientific Instruments
    ContactMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Year of Publishing2021
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