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Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
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SYSNO ASEP 0535590 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Ježek, Jan (UPT-D) RID, ORCID, SAI
Kizovský, Martin (UPT-D)
Klementová, Tereza (UPT-D) ORCID, SAI
Krátký, Stanislav (UPT-D) RID, ORCID, SAI
Sobota, Jaroslav (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Buryška, T. (CZ)
Damborský, J. (CZ)
Prokop, Z. (CZ)Number of authors 11 Source Title PhotonIcs and Electromagnetics Research Symposium - Spring, PIERS-Spring 2019. Proceedings. - New York : IEEE, 2019 - ISSN 1559-9450 - ISBN 978-1-7281-3403-1 Pages s. 1301-1309 Number of pages 9 s. Publication form Print - P Action PhotonIcs and Electromagnetics Research Symposium - Spring (PIERS-Spring) 2019 Event date 17.06.2019 - 20.06.2019 VEvent location Rome Country IT - Italy Event type WRD Language eng - English Country US - United States Keywords chemical analysis ; Raman spectroscopy ; emission spectroscopy ; light transmission Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GA16-07965S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000550769301050 EID SCOPUS 85082018253 Annotation Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2021
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