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Microtubule Cytoskeleton Remodeling by Nanosecond Pulsed Electric Fields

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    SYSNO ASEP0534878
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleMicrotubule Cytoskeleton Remodeling by Nanosecond Pulsed Electric Fields
    Author(s) Chafai, Djamel Eddine (URE-Y)
    Vostárek, František (FGU-C) RID, ORCID
    Dráberová, Eduarda (UMG-J) RID
    Havelka, Daniel (URE-Y) RID
    Arnaud-Cormos, D. (FR)
    Leveque, Ph. (FR)
    Janáček, Jiří (FGU-C) RID, ORCID
    Kubínová, Lucie (FGU-C) RID, ORCID
    Cifra, Michal (URE-Y) RID
    Dráber, Pavel (UMG-J) RID
    Number of authors10
    Article number2000070
    Source TitleAdvanced Biosystems
    Roč. 4, č. 7 (2020)
    Number of pages6 s.
    Publication formOnline - E
    Languageeng - English
    CountryDE - Germany
    Keywordsbioinspired materials ; cytoskeleton remodeling ; end-binding proteins ; microtubules ; nanosecond pulsed electric fields
    Subject RIVJA - Electronics ; Optoelectronics, Electrical Engineering
    OECD categoryElectrical and electronic engineering
    Subject RIV - cooperationInstitute of Physiology - Cell Biology
    Institute of Molecular Genetics - Cell Biology
    R&D ProjectsGA17-11898S GA ČR - Czech Science Foundation (CSF)
    GA18-23597S GA ČR - Czech Science Foundation (CSF)
    LM2018129 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    LTAUSA19118 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportURE-Y - RVO:67985882 ; FGU-C - RVO:67985823 ; UMG-J - RVO:68378050
    UT WOS000535572000001
    EID SCOPUS85085549314
    DOI10.1002/adbi.202000070
    AnnotationRemodeling of nanoscopic structures is not just crucial for cell biology, but it is also at the core of bioinspired materials. While the microtubule cytoskeleton in cells undergoes fast adaptation, adaptive materials still face this remodeling challenge. Moreover, the guided reorganization of the microtubule network and the correction of its abnormalities is still a major aim. This work reports new findings for externally triggered microtubule network remodeling by nanosecond electropulses (nsEPs). At first, a wide range of nsEP parameters, applied in a low conductivity buffer, is explored to find out the minimal nsEP dosage needed to disturb microtubules in various cell types. The time course of apoptosis and microtubule recovery in the culture medium is thereafter assessed. Application of nsEPs to cells in culture media result in modulation of microtubule binding properties to end-binding (EB1) protein, quantified by newly developed image processing techniques. The microtubules in nsEP-treated cells in the culture medium have longer EB1 comets but their density is lower than that of the control. The nsEP treatment represents a strategy for microtubule remodeling-based nano-biotechnological applications, such as engineering of self-healing materials, and as a manipulation tool for the evaluation of microtubule remodeling mechanisms during various biological processes in health and disease
    WorkplaceInstitute of Radio Engineering and Electronics
    ContactPetr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488
    Year of Publishing2021
    Electronic addresshttps://doi.org/10.1002/adbi.202000070
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