Number of the records: 1

Microtubule Cytoskeleton Remodeling by Nanosecond Pulsed Electric Fields

SYSNO ASEP	0534878
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Microtubule Cytoskeleton Remodeling by Nanosecond Pulsed Electric Fields
Author(s)	Chafai, Djamel Eddine (URE-Y) Vostárek, František (FGU-C)_{RID, ORCID, SAI} Dráberová, Eduarda (UMG-J)_{RID, ORCID} Havelka, Daniel (URE-Y)_RID Arnaud-Cormos, D. (FR) Leveque, Ph. (FR) Janáček, Jiří (FGU-C)_{RID, ORCID} Kubínová, Lucie (FGU-C)_{RID, ORCID} Cifra, Michal (URE-Y)_{RID, ORCID, SAI} Dráber, Pavel (UMG-J)_{RID, ORCID}
Number of authors	10
Article number	2000070
Source Title	Advanced Biosystems. - : Wiley Roč. 4, č. 7 (2020)
Number of pages	6 s.
Publication form	Online - E
Language	eng - English
Country	DE - Germany
Keywords	bioinspired materials ; cytoskeleton remodeling ; end-binding proteins ; microtubules ; nanosecond pulsed electric fields
Subject RIV	JA - Electronics ; Optoelectronics, Electrical Engineering
OECD category	Electrical and electronic engineering
Subject RIV - cooperation	Institute of Physiology - Cell Biology Institute of Molecular Genetics - Cell Biology
R&D Projects	GA17-11898S GA ČR - Czech Science Foundation (CSF)
	GA18-23597S GA ČR - Czech Science Foundation (CSF)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTAUSA19118 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	URE-Y - RVO:67985882 ; FGU-C - RVO:67985823 ; UMG-J - RVO:68378050
UT WOS	000535572000001
EID SCOPUS	85085549314
DOI	10.1002/adbi.202000070
Annotation	Remodeling of nanoscopic structures is not just crucial for cell biology, but it is also at the core of bioinspired materials. While the microtubule cytoskeleton in cells undergoes fast adaptation, adaptive materials still face this remodeling challenge. Moreover, the guided reorganization of the microtubule network and the correction of its abnormalities is still a major aim. This work reports new findings for externally triggered microtubule network remodeling by nanosecond electropulses (nsEPs). At first, a wide range of nsEP parameters, applied in a low conductivity buffer, is explored to find out the minimal nsEP dosage needed to disturb microtubules in various cell types. The time course of apoptosis and microtubule recovery in the culture medium is thereafter assessed. Application of nsEPs to cells in culture media result in modulation of microtubule binding properties to end-binding (EB1) protein, quantified by newly developed image processing techniques. The microtubules in nsEP-treated cells in the culture medium have longer EB1 comets but their density is lower than that of the control. The nsEP treatment represents a strategy for microtubule remodeling-based nano-biotechnological applications, such as engineering of self-healing materials, and as a manipulation tool for the evaluation of microtubule remodeling mechanisms during various biological processes in health and disease
Workplace	Institute of Radio Engineering and Electronics
Contact	Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488
Year of Publishing	2021
Electronic address	https://doi.org/10.1002/adbi.202000070

Number of the records: 1