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Microtubule Cytoskeleton Remodeling by Nanosecond Pulsed Electric Fields
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SYSNO ASEP 0534878 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microtubule Cytoskeleton Remodeling by Nanosecond Pulsed Electric Fields Author(s) Chafai, Djamel Eddine (URE-Y)
Vostárek, František (FGU-C) RID, ORCID, SAI
Dráberová, Eduarda (UMG-J) RID, ORCID
Havelka, Daniel (URE-Y) RID
Arnaud-Cormos, D. (FR)
Leveque, Ph. (FR)
Janáček, Jiří (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCID
Cifra, Michal (URE-Y) RID, ORCID, SAI
Dráber, Pavel (UMG-J) RID, ORCIDNumber of authors 10 Article number 2000070 Source Title Advanced Biosystems. - : Wiley
Roč. 4, č. 7 (2020)Number of pages 6 s. Publication form Online - E Language eng - English Country DE - Germany Keywords bioinspired materials ; cytoskeleton remodeling ; end-binding proteins ; microtubules ; nanosecond pulsed electric fields Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Electrical and electronic engineering Subject RIV - cooperation Institute of Physiology - Cell Biology
Institute of Molecular Genetics - Cell BiologyR&D Projects GA17-11898S GA ČR - Czech Science Foundation (CSF) GA18-23597S GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTAUSA19118 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support URE-Y - RVO:67985882 ; FGU-C - RVO:67985823 ; UMG-J - RVO:68378050 UT WOS 000535572000001 EID SCOPUS 85085549314 DOI 10.1002/adbi.202000070 Annotation Remodeling of nanoscopic structures is not just crucial for cell biology, but it is also at the core of bioinspired materials. While the microtubule cytoskeleton in cells undergoes fast adaptation, adaptive materials still face this remodeling challenge. Moreover, the guided reorganization of the microtubule network and the correction of its abnormalities is still a major aim. This work reports new findings for externally triggered microtubule network remodeling by nanosecond electropulses (nsEPs). At first, a wide range of nsEP parameters, applied in a low conductivity buffer, is explored to find out the minimal nsEP dosage needed to disturb microtubules in various cell types. The time course of apoptosis and microtubule recovery in the culture medium is thereafter assessed. Application of nsEPs to cells in culture media result in modulation of microtubule binding properties to end-binding (EB1) protein, quantified by newly developed image processing techniques. The microtubules in nsEP-treated cells in the culture medium have longer EB1 comets but their density is lower than that of the control. The nsEP treatment represents a strategy for microtubule remodeling-based nano-biotechnological applications, such as engineering of self-healing materials, and as a manipulation tool for the evaluation of microtubule remodeling mechanisms during various biological processes in health and disease Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2021 Electronic address https://doi.org/10.1002/adbi.202000070
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