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Analysis of Bacteriophage-Host Interaction by Raman Tweezers
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SYSNO ASEP 0534715 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of Bacteriophage-Host Interaction by Raman Tweezers Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
Pilátová, J. (CZ)
Klementová, Tereza (UPT-D) ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Maňka, Tadeáš (UPT-D) RID, ORCID, SAI
Kizovský, Martin (UPT-D)
Růžička, F. (CZ)
Pantůček, R. (CZ)
Neugebauer, U. (DE)
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 13 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 92, č. 18 (2020), s. 12304-12311Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords staphylococcus-aureus ; electron-transport ; virus structure ; spectroscopy Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GA19-20697S GA ČR - Czech Science Foundation (CSF) GF19-29651L GA ČR - Czech Science Foundation (CSF) EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UPT-D - RVO:68081731 UT WOS 000572832900029 EID SCOPUS 85094979024 DOI 10.1021/acs.analchem.0c01963 Annotation Bacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2021 Electronic address https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963
Number of the records: 1