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Analysis of Bacteriophage-Host Interaction by Raman Tweezers

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    SYSNO ASEP0534715
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleAnalysis of Bacteriophage-Host Interaction by Raman Tweezers
    Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
    Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
    Pilátová, J. (CZ)
    Klementová, Tereza (UPT-D) ORCID, SAI
    Bernatová, Silvie (UPT-D) RID, SAI, ORCID
    Šiler, Martin (UPT-D) RID, ORCID, SAI
    Maňka, Tadeáš (UPT-D) RID, ORCID, SAI
    Kizovský, Martin (UPT-D)
    Růžička, F. (CZ)
    Pantůček, R. (CZ)
    Neugebauer, U. (DE)
    Samek, Ota (UPT-D) RID, ORCID, SAI
    Zemánek, Pavel (UPT-D) RID, SAI, ORCID
    Number of authors13
    Source TitleAnalytical Chemistry - ISSN 0003-2700
    Roč. 92, č. 18 (2020), s. 12304-12311
    Number of pages8 s.
    Publication formPrint - P
    Languageeng - English
    CountryUS - United States
    Keywordsstaphylococcus-aureus ; electron-transport ; virus structure ; spectroscopy
    Subject RIVBH - Optics, Masers, Lasers
    OECD categoryOptics (including laser optics and quantum optics)
    R&D ProjectsGA19-20697S GA ČR - Czech Science Foundation (CSF)
    GF19-29651L GA ČR - Czech Science Foundation (CSF)
    EF15_003/0000476 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportUPT-D - RVO:68081731
    UT WOS000572832900029
    EID SCOPUS85094979024
    DOI10.1021/acs.analchem.0c01963
    AnnotationBacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
    WorkplaceInstitute of Scientific Instruments
    ContactMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Year of Publishing2021
    Electronic addresshttps://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963
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